6PC2

Crystal structure of Helicobacter pylori PPX/GppA in complex with GNP

6PC2 の概要

• エントリーDOI: 10.2210/pdb6pc2/pdb
• 分子名称: Guanosine pentaphosphate phosphohydrolase, MAGNESIUM ION, PHOSPHATE ION, ... (5 entities in total)
• 機能のキーワード: pppgpp, ppgpp, ppx, gppa, polyphosphate, hydrolase
• 由来する生物種: Helicobacter pylori (strain G27)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 112832.59

構造登録者

Song, H.,Wang, C.,Shaw, G.X.,Ji, X. (登録日: 2019-06-15, 公開日: 2019-11-20, 最終更新日: 2023-10-11)

主引用文献

Song, H.,Dharmasena, M.N.,Wang, C.,Shaw, G.X.,Cherry, S.,Tropea, J.E.,Jin, D.J.,Ji, X.
Structure and activity of PPX/GppA homologs from Escherichia coli and Helicobacter pylori.
Febs J., 287:1865-1885, 2020

PubMed Abstract: Rapid adaptation to environmental changes is crucial for bacterial survival. Almost all bacteria possess a conserved stringent response system to prompt transcriptional and metabolic responses toward stress. The adaptive process relies on alarmones, guanosine pentaphosphate (pppGpp), and tetraphosphate (ppGpp), to regulate global gene expression. The ppGpp is more potent than pppGpp in the regulatory activity, and pppGpp phosphohydrolase (GppA) plays a key role in (p)ppGpp homeostasis. Sharing a similar domain structure, GppA is indistinguishable from exopolyphosphatase (PPX), which mediates the metabolism of cellular inorganic polyphosphate. Here, our phylogenetic analysis of PPX/GppA homologs in bacteria shows a wide distribution with several distinct subfamilies, and our structural and functional analysis of Escherichia coli GppA and Helicobacter pylori PPX/GppA reveals unique properties of each homolog. These results explain how each homolog possesses its distinct functionality.

PubMed: 31679177
DOI: 10.1111/febs.15120

実験手法

X-RAY DIFFRACTION (2.9 Å)