6PBB

Crystal structure of Hen Egg White Lysozyme in complex with I3C

6PBB の概要

• エントリーDOI: 10.2210/pdb6pbb/pdb
• 分子名称: Lysozyme, 5-amino-2,4,6-triiodobenzene-1,3-dicarboxylic acid, CHLORIDE ION, ... (4 entities in total)
• 機能のキーワード: hydrolase
• 由来する生物種: Gallus gallus (Chicken)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 16601.95

構造登録者

Truong, J.Q. (登録日: 2019-06-13, 公開日: 2019-07-10, 最終更新日: 2024-10-16)

主引用文献

Truong, J.Q.,Panjikar, S.,Shearwin-Whyatt, L.,Bruning, J.B.,Shearwin, K.E.
Combining random microseed matrix screening and the magic triangle for the efficient structure solution of a potential lysin from bacteriophage P68.
Acta Crystallogr D Struct Biol, 75:670-681, 2019

PubMed Abstract: Two commonly encountered bottlenecks in the structure determination of a protein by X-ray crystallography are screening for conditions that give high-quality crystals and, in the case of novel structures, finding derivatization conditions for experimental phasing. In this study, the phasing molecule 5-amino-2,4,6-triiodoisophthalic acid (I3C) was added to a random microseed matrix screen to generate high-quality crystals derivatized with I3C in a single optimization experiment. I3C, often referred to as the magic triangle, contains an aromatic ring scaffold with three bound I atoms. This approach was applied to efficiently phase the structures of hen egg-white lysozyme and the N-terminal domain of the Orf11 protein from Staphylococcus phage P68 (Orf11 NTD) using SAD phasing. The structure of Orf11 NTD suggests that it may play a role as a virion-associated lysin or endolysin.

PubMed: 31282476
DOI: 10.1107/S2059798319009008

実験手法

X-RAY DIFFRACTION (1.89151078799 Å)