6PBB
Crystal structure of Hen Egg White Lysozyme in complex with I3C
6PBB の概要
エントリーDOI | 10.2210/pdb6pbb/pdb |
分子名称 | Lysozyme, 5-amino-2,4,6-triiodobenzene-1,3-dicarboxylic acid, CHLORIDE ION, ... (4 entities in total) |
機能のキーワード | hydrolase |
由来する生物種 | Gallus gallus (Chicken) |
タンパク質・核酸の鎖数 | 1 |
化学式量合計 | 16601.95 |
構造登録者 | |
主引用文献 | Truong, J.Q.,Panjikar, S.,Shearwin-Whyatt, L.,Bruning, J.B.,Shearwin, K.E. Combining random microseed matrix screening and the magic triangle for the efficient structure solution of a potential lysin from bacteriophage P68. Acta Crystallogr D Struct Biol, 75:670-681, 2019 Cited by PubMed Abstract: Two commonly encountered bottlenecks in the structure determination of a protein by X-ray crystallography are screening for conditions that give high-quality crystals and, in the case of novel structures, finding derivatization conditions for experimental phasing. In this study, the phasing molecule 5-amino-2,4,6-triiodoisophthalic acid (I3C) was added to a random microseed matrix screen to generate high-quality crystals derivatized with I3C in a single optimization experiment. I3C, often referred to as the magic triangle, contains an aromatic ring scaffold with three bound I atoms. This approach was applied to efficiently phase the structures of hen egg-white lysozyme and the N-terminal domain of the Orf11 protein from Staphylococcus phage P68 (Orf11 NTD) using SAD phasing. The structure of Orf11 NTD suggests that it may play a role as a virion-associated lysin or endolysin. PubMed: 31282476DOI: 10.1107/S2059798319009008 主引用文献が同じPDBエントリー |
実験手法 | X-RAY DIFFRACTION (1.89151078799 Å) |
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