6P59

Crystal structure of SIVrcm Vif-CBFbeta-ELOB-ELOC complex

6P59 の概要

• エントリーDOI: 10.2210/pdb6p59/pdb
• 分子名称: Core-binding factor subunit beta, Elongin-B, Elongin-C, ... (8 entities in total)
• 機能のキーワード: complex, vif, a3g, viral protein, viral protein-immune system complex, viral protein/immune system
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 142429.32

構造登録者

Binning, J.M.,Chesarino, N.M.,Emerman, M.,Gross, J.D. (登録日: 2019-05-29, 公開日: 2019-12-25, 最終更新日: 2023-10-11)

主引用文献

Binning, J.M.,Chesarino, N.M.,Emerman, M.,Gross, J.D.
Structural Basis for a Species-Specific Determinant of an SIV Vif Protein toward Hominid APOBEC3G Antagonism.
Cell Host Microbe, 26:739-747.e4, 2019

PubMed Abstract: Primate lentiviruses encode a Vif protein that counteracts the host antiviral APOBEC3 (A3) family members. The adaptation of Vif to species-specific A3 determinants is a critical event that allowed the spillover of a lentivirus from monkey reservoirs to chimpanzees and subsequently to humans, which gave rise to HIV-1 and the acquired immune deficiency syndrome (AIDS) pandemic. How Vif-A3 protein interactions are remodeled during evolution is unclear. Here, we report a 2.94 Å crystal structure of the Vif substrate receptor complex from simian immunodeficiency virus isolated from red-capped mangabey (SIVrcm). The structure of the SIVrcm Vif complex illuminates the stage of lentiviral Vif evolution that is immediately prior to entering hominid primates. Structure-function studies reveal the adaptations that allowed SIVrcm Vif to antagonize hominid A3G. These studies show a partitioning between an evolutionarily dynamic specificity determinant and a conserved protein interacting surface on Vif that enables adaptation while maintaining protein interactions required for potent A3 antagonism.

PubMed: 31830442
DOI: 10.1016/j.chom.2019.10.014

実験手法

X-RAY DIFFRACTION (2.94221405643 Å)