6OX4

A SETD3 Mutant (N255A) in Complex with an Actin Peptide

6OX4 の概要

• エントリーDOI: 10.2210/pdb6ox4/pdb
• 分子名称: Actin Peptide, Actin-histidine N-methyltransferase, 1,2-ETHANEDIOL, ... (7 entities in total)
• 機能のキーワード: transferase, transferase-structural protein complex, transferase/structural protein
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 142146.35

構造登録者

Horton, J.R.,Dai, S.,Cheng, X. (登録日: 2019-05-13, 公開日: 2019-08-21, 最終更新日: 2023-10-11)

主引用文献

Dai, S.,Horton, J.R.,Woodcock, C.B.,Wilkinson, A.W.,Zhang, X.,Gozani, O.,Cheng, X.
Structural basis for the target specificity of actin histidine methyltransferase SETD3.
Nat Commun, 10:3541-3541, 2019

PubMed Abstract: SETD3 is an actin histidine-N methyltransferase, whereas other characterized SET-domain enzymes are protein lysine methyltransferases. We report that in a pre-reactive complex SETD3 binds the N-protonated form (N-H) of actin His73, and in a post-reactive product complex, SETD3 generates the methylated histidine in an N-protonated (N-H) and N-methylated form. During the reaction, the imidazole ring of His73 rotates ~105°, which shifts the proton from N to N, thus ensuring that the target atom N is deprotonated prior to the methyl transfer. Under the conditions optimized for lysine deprotonation, SETD3 has weak lysine methylation activity on an actin peptide in which the target His73 is substituted by a lysine. The structure of SETD3 with Lys73-containing peptide reveals a bent conformation of Lys73, with its side chain aliphatic carbons tracing along the edge of imidazole ring and the terminal ε-amino group occupying a position nearly identical to the N atom of unmethylated histidine.

PubMed: 31388018
DOI: 10.1038/s41467-019-11554-6

実験手法

X-RAY DIFFRACTION (2.294 Å)