6OV6

CRYSTALLOGRAPHIC STRUCTURE OF THE C24 PROTEIN FROM THE ANTARCTIC MICROORGANISM BIZIONIA ARGENTINENSIS

Summary for 6OV6

• Entry DOI: 10.2210/pdb6ov6/pdb
• Descriptor: C24 PROTEIN, MANGANESE (II) ION (3 entities in total)
• Functional Keywords: fiber, viral origin, unknown function
• Biological source: Bizionia argentinensis JUB59
• Total number of polymer chains: 3
• Total formula weight: 89244.30

Authors

Klinke, S.,Rinaldi, J.,Guimaraes, B.G.,Pellizza, L.,Aran, M. (deposition date: 2019-05-07, release date: 2020-08-05, Last modification date: 2024-03-13)

Primary citation

Pellizza, L.,Lopez, J.L.,Vazquez, S.,Sycz, G.,Guimaraes, B.G.,Rinaldi, J.,Goldbaum, F.A.,Aran, M.,Mac Cormack, W.P.,Klinke, S.
Structure of the putative long tail fiber receptor-binding tip of a novel temperate bacteriophage from the Antarctic bacterium Bizionia argentinensis JUB59.
J.Struct.Biol., 212:107595-107595, 2020

PubMed Abstract: Tailed bacteriophages are one of the most widespread biological entities on Earth. Their singular structures, such as spikes or fibers are of special interest given their potential use in a wide range of biotechnological applications. In particular, the long fibers present at the termini of the T4 phage tail have been studied in detail and are important for host recognition and adsorption. Although significant progress has been made in elucidating structural mechanisms of model phages, the high-resolution structural description of the vast population of marine phages is still unexplored. In this context, we present here the crystal structure of C24, a putative receptor-binding tip-like protein from Bizionia argentinensis JUB59, a psychrotolerant bacterium isolated from the marine surface waters of Potter Cove, Antarctica. The structure resembles the receptor-binding tip from the bacteriophage T4 long tail fiber yet showing marked differences in its domain organization, size, sequence identity and metal binding nature. We confirmed the viral origin of C24 by induction experiments using mitomycin C. Our results reveal the presence of a novel uncharacterized prophage in the genome of B. argentinensis JUB59, whose morphology is compatible with the order Caudovirales and that carries the nucleotide sequence of C24 in its genome. This work provides valuable information to expand our current knowledge on the viral machinery prevalent in the oceans.

PubMed: 32736071
DOI: 10.1016/j.jsb.2020.107595

Experimental method

X-RAY DIFFRACTION (1.82 Å)