6OUA

Cryo-EM structure of the yeast Ctf3 complex

6OUA の概要

• エントリーDOI: 10.2210/pdb6oua/pdb
• 関連するPDBエントリー: 6NUW
• EMDBエントリー: 0523 20200
• 分子名称: Inner kinetochore subunit CTF3, Inner kinetochore subunit MCM16, Inner kinetochore subunit MCM22 (3 entities in total)
• 機能のキーワード: kinetochore, chromosome, cryo-em, cell cycle
• 由来する生物種: Saccharomyces cerevisiae (Baker's yeast); 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 133931.05

構造登録者

Hinshaw, S.M.,Harrison, S.C. (登録日: 2019-05-04, 公開日: 2019-05-15, 最終更新日: 2025-06-04)

主引用文献

Hinshaw, S.M.,Dates, A.N.,Harrison, S.C.
The structure of the yeast Ctf3 complex.
Elife, 8:-, 2019

PubMed Abstract: Kinetochores are the chromosomal attachment points for spindle microtubules. They are also signaling hubs that control major cell cycle transitions and coordinate chromosome folding. Most well-studied eukaryotes rely on a conserved set of factors, which are divided among two loosely-defined groups, for these functions. Outer kinetochore proteins contact microtubules or regulate this contact directly. Inner kinetochore proteins designate the kinetochore assembly site by recognizing a specialized nucleosome containing the H3 variant Cse4/CENP-A. We previously determined the structure, resolved by cryo-electron microscopy (cryo-EM), of the yeast Ctf19 complex (Ctf19c, homologous to the vertebrate CCAN), providing a high-resolution view of inner kinetochore architecture (Hinshaw and Harrison, 2019). We now extend these observations by reporting a near-atomic model of the Ctf3 complex, the outermost Ctf19c sub-assembly seen in our original cryo-EM density. The model is sufficiently well-determined by the new data to enable molecular interpretation of Ctf3 recruitment and function.

PubMed: 31194673
DOI: 10.7554/eLife.48215

実験手法

ELECTRON MICROSCOPY (4.18 Å)