6OR2

MmpL3 is a lipid transporter that binds trehalose monomycolate and phosphatidylethanolamine

「6N3T」から置き換えられました

6OR2 の概要

• エントリーDOI: 10.2210/pdb6or2/pdb
• 関連するPDBエントリー: 6N40
• 分子名称: Membrane protein, MmpL family protein, (1S)-2-{[(S)-(2-aminoethoxy)(hydroxy)phosphoryl]oxy}-1-[(octadecanoyloxy)methyl]ethyl (9Z)-octadec-9-enoate, DODECYL-BETA-D-MALTOSIDE, ... (4 entities in total)
• 機能のキーワード: transporter, membrane protein
• 由来する生物種: Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 86699.00

構造登録者

Su, C.-C. (登録日: 2019-04-29, 公開日: 2019-05-29, 最終更新日: 2024-03-13)

主引用文献

Su, C.C.,Klenotic, P.A.,Bolla, J.R.,Purdy, G.E.,Robinson, C.V.,Yu, E.W.
MmpL3 is a lipid transporter that binds trehalose monomycolate and phosphatidylethanolamine.
Proc.Natl.Acad.Sci.USA, 116:11241-11246, 2019

PubMed Abstract: The cell envelope of is notable for the abundance of mycolic acids (MAs), essential to mycobacterial viability, and of other species-specific lipids. The mycobacterial cell envelope is extremely hydrophobic, which contributes to virulence and antibiotic resistance. However, exactly how fatty acids and lipidic elements are transported across the cell envelope for cell-wall biosynthesis is unclear. Mycobacterial membrane protein Large 3 (MmpL3) is essential and required for transport of trehalose monomycolates (TMMs), precursors of MA-containing trehalose dimycolates (TDM) and mycolyl arabinogalactan peptidoglycan, but the exact function of MmpL3 remains elusive. Here, we report a crystal structure of MmpL3 at a resolution of 2.59 Å, revealing a monomeric molecule that is structurally distinct from all known bacterial membrane proteins. A previously unknown MmpL3 ligand, phosphatidylethanolamine (PE), was discovered inside this transporter. We also show, via native mass spectrometry, that MmpL3 specifically binds both TMM and PE, but not TDM, in the micromolar range. These observations provide insight into the function of MmpL3 and suggest a possible role for this protein in shuttling a variety of lipids to strengthen the mycobacterial cell wall.

PubMed: 31113875
DOI: 10.1073/pnas.1901346116

実験手法

X-RAY DIFFRACTION (2.59 Å)