6OLY

Full-length MthK channel at 3.1 angstrom resolution

6OLY の概要

• エントリーDOI: 10.2210/pdb6oly/pdb
• 分子名称: Calcium-gated potassium channel MthK, CALCIUM ION (3 entities in total)
• 機能のキーワード: potassium channel, calcium-binding, rck domain, rossmann fold, membrane protein
• 由来する生物種: Methanothermobacter thermautotrophicus
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 155591.83

構造登録者

Rothberg, B.S. (登録日: 2019-04-17, 公開日: 2019-12-11, 最終更新日: 2023-10-11)

主引用文献

Kopec, W.,Rothberg, B.S.,de Groot, B.L.
Molecular mechanism of a potassium channel gating through activation gate-selectivity filter coupling.
Nat Commun, 10:5366-5366, 2019

PubMed Abstract: Potassium channels are presumed to have two allosterically coupled gates, the activation gate and the selectivity filter gate, that control channel opening, closing, and inactivation. However, the molecular mechanism of how these gates regulate Kion flow through the channel remains poorly understood. An activation process, occurring at the selectivity filter, has been recently proposed for several potassium channels. Here, we use X-ray crystallography and extensive molecular dynamics simulations, to study ion permeation through a potassium channel MthK, for various opening levels of both gates. We find that the channel conductance is controlled at the selectivity filter, whose conformation depends on the activation gate. The crosstalk between the gates is mediated through a collective motion of channel helices, involving hydrophobic contacts between an isoleucine and a conserved threonine in the selectivity filter. We propose a gating model of selectivity filter-activated potassium channels, including pharmacologically relevant two-pore domain (K2P) and big potassium (BK) channels.

PubMed: 31772184
DOI: 10.1038/s41467-019-13227-w

実験手法

X-RAY DIFFRACTION (3.112 Å)