6OJN

Comparative Model of SGIV Major Coat Protein (MCP) Trimer Based on Cryo-EM Map

6OJN の概要

• エントリーDOI: 10.2210/pdb6ojn/pdb
• EMDBエントリー: 20091
• 分子名称: Major capsid protein (1 entity in total)
• 機能のキーワード: icosahedral, trimers, outer coat, anchor proteins, zip proteins, viral protein
• 由来する生物種: Singapore grouper iridovirus
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 151719.42

構造登録者

Pintilie, G.,Chen, D.-H.,Tran, B.N.,Jakana, J.,Wu, J.,Hew, C.L.,Chiu, W. (登録日: 2019-04-11, 公開日: 2019-06-12, 最終更新日: 2024-03-20)

主引用文献

Pintilie, G.,Chen, D.H.,Tran, B.N.,Jakana, J.,Wu, J.,Hew, C.L.,Chiu, W.
Segmentation and Comparative Modeling in an 8.6- angstrom Cryo-EM Map of the Singapore Grouper Iridovirus.
Structure, 27:1561-, 2019

PubMed Abstract: SGIV, or Singapore grouper iridovirus, is a large double-stranded DNA virus, reaching a diameter of 220 nm and packaging a genome of 140 kb. We present a 3D cryoelectron microscopy (cryo-EM) icosahedral reconstruction of SGIV determined at 8.6-Å resolution. It reveals several layers including a T = 247 icosahedral outer coat, anchor proteins, a lipid bilayer, and the encapsidated DNA. A new segmentation tool, iSeg, was applied to extract these layers from the reconstructed map. The outer coat was further segmented into major and minor capsid proteins. None of the proteins extracted by segmentation have known atomic structures. We generated models for the major coat protein using three comparative modeling tools, and evaluated each model using the cryo-EM map. Our analysis reveals a new architecture in the Iridoviridae family of viruses. It shares similarities with others in the same family, e.g., Chilo iridescent virus, but also shows new features of the major and minor capsid proteins.

PubMed: 31447288
DOI: 10.1016/j.str.2019.08.002

実験手法

ELECTRON MICROSCOPY (8.6 Å)