6OIK

Muscarinic acetylcholine receptor 2-Go complex

6OIK の概要

• エントリーDOI: 10.2210/pdb6oik/pdb
• EMDBエントリー: 20079
• 分子名称: Muscarinic acetylcholine receptor M2,Muscarinic acetylcholine receptor M2, Guanine nucleotide-binding protein G(o) subunit alpha, Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1, ... (7 entities in total)
• 機能のキーワード: g-protein coupled receptor-g-protein complex, neurotransmitter receptor, signaling protein
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 153399.44

構造登録者

Maeda, S.,Qianhui, Q.,Skiniotis, G.,Kobilka, B. (登録日: 2019-04-09, 公開日: 2019-05-08, 最終更新日: 2024-11-20)

主引用文献

Maeda, S.,Qu, Q.,Robertson, M.J.,Skiniotis, G.,Kobilka, B.K.
Structures of the M1 and M2 muscarinic acetylcholine receptor/G-protein complexes.
Science, 364:552-557, 2019

PubMed Abstract: Muscarinic acetylcholine receptors are G protein-coupled receptors that respond to acetylcholine and play important signaling roles in the nervous system. There are five muscarinic receptor subtypes (M1R to M5R), which, despite sharing a high degree of sequence identity in the transmembrane region, couple to different heterotrimeric GTP-binding proteins (G proteins) to transmit signals. M1R, M3R, and M5R couple to the G family, whereas M2R and M4R couple to the G family. Here, we present and compare the cryo-electron microscopy structures of M1R in complex with G and M2R in complex with G The M1R-G complex exhibits distinct features, including an extended transmembrane helix 5 and carboxyl-terminal receptor tail that interacts with G protein. Detailed analysis of these structures provides a framework for understanding the molecular determinants of G-protein coupling selectivity.

PubMed: 31073061
DOI: 10.1126/science.aaw5188

実験手法

ELECTRON MICROSCOPY (3.6 Å)