6OHW

Structural basis for human coronavirus attachment to sialic acid receptors. Apo-HCoV-OC43 S

6OHW の概要

• エントリーDOI: 10.2210/pdb6ohw/pdb
• EMDBエントリー: 0557 20070
• 分子名称: Spike surface glycoprotein, beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (6 entities in total)
• 機能のキーワード: coronavirus spike glycoprotein, sialic acid, hcov-oc43, fusion protein, viral protein
• 由来する生物種: Human coronavirus OC43 (HCoV-OC43)
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 458770.04

構造登録者

Tortorici, M.A.,Walls, A.C.,Lang, Y.,Wang, C.,Li, Z.,Koerhuis, D.,Boons, G.J.,Bosch, B.J.,Rey, F.A.,de Groot, R.,Veesler, D. (登録日: 2019-04-07, 公開日: 2019-06-05, 最終更新日: 2024-11-06)

主引用文献

Alejandra Tortorici, M.,Walls, A.C.,Lang, Y.,Wang, C.,Li, Z.,Koerhuis, D.,Boons, G.J.,Bosch, B.J.,Rey, F.A.,de Groot, R.J.,Veesler, D.
Structural basis for human coronavirus attachment to sialic acid receptors.
Nat.Struct.Mol.Biol., 26:481-489, 2019

PubMed Abstract: Coronaviruses cause respiratory tract infections in humans and outbreaks of deadly pneumonia worldwide. Infections are initiated by the transmembrane spike (S) glycoprotein, which binds to host receptors and fuses the viral and cellular membranes. To understand the molecular basis of coronavirus attachment to oligosaccharide receptors, we determined cryo-EM structures of coronavirus OC43 S glycoprotein trimer in isolation and in complex with a 9-O-acetylated sialic acid. We show that the ligand binds with fast kinetics to a surface-exposed groove and that interactions at the identified site are essential for S-mediated viral entry into host cells, but free monosaccharide does not trigger fusogenic conformational changes. The receptor-interacting site is conserved in all coronavirus S glycoproteins that engage 9-O-acetyl-sialogycans, with an architecture similar to those of the ligand-binding pockets of coronavirus hemagglutinin esterases and influenza virus C/D hemagglutinin-esterase fusion glycoproteins. Our results demonstrate these viruses evolved similar strategies to engage sialoglycans at the surface of target cells.

PubMed: 31160783
DOI: 10.1038/s41594-019-0233-y

実験手法

ELECTRON MICROSCOPY (2.9 Å)