6OGZ
In situ structure of Rotavirus RNA-dependent RNA polymerase at transcript-elongated state
Summary for 6OGZ
Entry DOI | 10.2210/pdb6ogz/pdb |
EMDB information | 20059 20060 |
Descriptor | RNA (5'-R(P*UP*AP*UP*AP*UP*AP*UP*AP*UP*AP*UP*AP*UP*AP*UP*AP*UP*A)-3'), RNA (5'-R(P*AP*UP*AP*UP*AP*UP*AP*UP*AP*UP*AP*UP*AP*UP*AP*UP*A)-3'), RNA-dependent RNA polymerase of rotavirus A, ... (6 entities in total) |
Functional Keywords | rna-dependent rna polymerase, capsid shell protein, transcription, in situ structure, rotavirus, transcriptional factors, reovirus, virus, viral protein-rna-transferase complex, viral protein/rna/transferase |
Biological source | Rotavirus A More |
Total number of polymer chains | 13 |
Total formula weight | 1171584.16 |
Authors | Ding, K.,Chang, T.,Shen, W.,Roy, P.,Zhou, Z.H. (deposition date: 2019-04-03, release date: 2019-05-22, Last modification date: 2025-05-21) |
Primary citation | Ding, K.,Celma, C.C.,Zhang, X.,Chang, T.,Shen, W.,Atanasov, I.,Roy, P.,Zhou, Z.H. In situ structures of rotavirus polymerase in action and mechanism of mRNA transcription and release. Nat Commun, 10:2216-2216, 2019 Cited by PubMed Abstract: Transcribing and replicating a double-stranded genome require protein modules to unwind, transcribe/replicate nucleic acid substrates, and release products. Here we present in situ cryo-electron microscopy structures of rotavirus dsRNA-dependent RNA polymerase (RdRp) in two states pertaining to transcription. In addition to the previously discovered universal "hand-shaped" polymerase core domain shared by DNA polymerases and telomerases, our results show the function of N- and C-terminal domains of RdRp: the former opens the genome duplex to isolate the template strand; the latter splits the emerging template-transcript hybrid, guides genome reannealing to form a transcription bubble, and opens a capsid shell protein (CSP) to release the transcript. These two "helicase" domains also extensively interact with CSP, which has a switchable N-terminal helix that, like cellular transcriptional factors, either inhibits or promotes RdRp activity. The in situ structures of RdRp, CSP, and RNA in action inform mechanisms of not only transcription, but also replication. PubMed: 31101900DOI: 10.1038/s41467-019-10236-7 PDB entries with the same primary citation |
Experimental method | ELECTRON MICROSCOPY (3.6 Å) |
Structure validation
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