6OGZ

In situ structure of Rotavirus RNA-dependent RNA polymerase at transcript-elongated state

6OGZ の概要

• エントリーDOI: 10.2210/pdb6ogz/pdb
• EMDBエントリー: 20059 20060
• 分子名称: RNA (5'-R(P*UP*AP*UP*AP*UP*AP*UP*AP*UP*AP*UP*AP*UP*AP*UP*AP*UP*A)-3'), RNA (5'-R(P*AP*UP*AP*UP*AP*UP*AP*UP*AP*UP*AP*UP*AP*UP*AP*UP*A)-3'), RNA-dependent RNA polymerase of rotavirus A, ... (6 entities in total)
• 機能のキーワード: rna-dependent rna polymerase, capsid shell protein, transcription, in situ structure, rotavirus, transcriptional factors, reovirus, virus, viral protein-rna-transferase complex, viral protein/rna/transferase
• 由来する生物種: Rotavirus A; 詳細
• タンパク質・核酸の鎖数: 13
• 化学式量合計: 1171584.16

構造登録者

Ding, K.,Chang, T.,Shen, W.,Roy, P.,Zhou, Z.H. (登録日: 2019-04-03, 公開日: 2019-05-22, 最終更新日: 2025-05-21)

主引用文献

Ding, K.,Celma, C.C.,Zhang, X.,Chang, T.,Shen, W.,Atanasov, I.,Roy, P.,Zhou, Z.H.
In situ structures of rotavirus polymerase in action and mechanism of mRNA transcription and release.
Nat Commun, 10:2216-2216, 2019

PubMed Abstract: Transcribing and replicating a double-stranded genome require protein modules to unwind, transcribe/replicate nucleic acid substrates, and release products. Here we present in situ cryo-electron microscopy structures of rotavirus dsRNA-dependent RNA polymerase (RdRp) in two states pertaining to transcription. In addition to the previously discovered universal "hand-shaped" polymerase core domain shared by DNA polymerases and telomerases, our results show the function of N- and C-terminal domains of RdRp: the former opens the genome duplex to isolate the template strand; the latter splits the emerging template-transcript hybrid, guides genome reannealing to form a transcription bubble, and opens a capsid shell protein (CSP) to release the transcript. These two "helicase" domains also extensively interact with CSP, which has a switchable N-terminal helix that, like cellular transcriptional factors, either inhibits or promotes RdRp activity. The in situ structures of RdRp, CSP, and RNA in action inform mechanisms of not only transcription, but also replication.

PubMed: 31101900
DOI: 10.1038/s41467-019-10236-7

実験手法

ELECTRON MICROSCOPY (3.6 Å)