6OFT

The crystal structure of the first half of the periplasmic protease PqqL from Escherichia coli

6OFT の概要

• エントリーDOI: 10.2210/pdb6oft/pdb
• 分子名称: Probable zinc protease PqqL, GLYCEROL, PHOSPHATE ION, ... (5 entities in total)
• 機能のキーワード: protease, m16 family, processing protease, hydrolase
• 由来する生物種: Escherichia coli (strain K12)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 108804.86

構造登録者

Grinter, R. (登録日: 2019-04-01, 公開日: 2019-10-02, 最終更新日: 2023-10-11)

主引用文献

Grinter, R.,Leung, P.M.,Wijeyewickrema, L.C.,Littler, D.,Beckham, S.,Pike, R.N.,Walker, D.,Greening, C.,Lithgow, T.
Protease-associated import systems are widespread in Gram-negative bacteria.
Plos Genet., 15:e1008435-e1008435, 2019

PubMed Abstract: Bacteria have evolved sophisticated uptake machineries in order to obtain the nutrients required for growth. Gram-negative plant pathogens of the genus Pectobacterium obtain iron from the protein ferredoxin, which is produced by their plant hosts. This iron-piracy is mediated by the ferredoxin uptake system (Fus), a gene cluster encoding proteins that transport ferredoxin into the bacterial cell and process it proteolytically. In this work we show that gene clusters related to the Fus are widespread in bacterial species. Through structural and biochemical characterisation of the distantly related Fus homologues YddB and PqqL from Escherichia coli, we show that these proteins are analogous to components of the Fus from Pectobacterium. The membrane protein YddB shares common structural features with the outer membrane ferredoxin transporter FusA, including a large extracellular substrate binding site. PqqL is an active protease with an analogous periplasmic localisation and iron-dependent expression to the ferredoxin processing protease FusC. Structural analysis demonstrates that PqqL and FusC share specific features that distinguish them from other members of the M16 protease family. Taken together, these data provide evidence that protease associated import systems analogous to the Fus are widespread in Gram-negative bacteria.

PubMed: 31613892
DOI: 10.1371/journal.pgen.1008435

実験手法

X-RAY DIFFRACTION (2 Å)