6OEV

Structure of human Patched1 in complex with native Sonic Hedgehog

「6D4J」から置き換えられました

6OEV の概要

• エントリーDOI: 10.2210/pdb6oev/pdb
• EMDBエントリー: 7796
• 分子名称: Protein patched homolog 1, Sonic hedgehog protein, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (5 entities in total)
• 機能のキーワード: tumor suppressor, hh, membrane protein
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 181903.48

構造登録者

Qi, X.,Li, X. (登録日: 2019-03-27, 公開日: 2019-04-17, 最終更新日: 2025-04-02)

主引用文献

Qi, X.,Schmiege, P.,Coutavas, E.,Wang, J.,Li, X.
Structures of human Patched and its complex with native palmitoylated sonic hedgehog.
Nature, 560:128-132, 2018

PubMed Abstract: Hedgehog (HH) signalling governs embryogenesis and adult tissue homeostasis in mammals and other multicellular organisms. Whereas deficient HH signalling leads to birth defects, unrestrained HH signalling is implicated in human cancers. N-terminally palmitoylated HH releases the repression of Patched to the oncoprotein smoothened (SMO); however, the mechanism by which HH recognizes Patched is unclear. Here we report cryo-electron microscopy structures of human patched 1 (PTCH1) alone and in complex with the N-terminal domain of 'native' sonic hedgehog (native SHH-N has both a C-terminal cholesterol and an N-terminal fatty-acid modification), at resolutions of 3.5 Å and 3.8 Å, respectively. The structure of PTCH1 has internal two-fold pseudosymmetry in the transmembrane core, which features a sterol-sensing domain and two homologous extracellular domains, resembling the architecture of Niemann-Pick C1 (NPC1) protein. The palmitoylated N terminus of SHH-N inserts into a cavity between the extracellular domains of PTCH1 and dominates the PTCH1-SHH-N interface, which is distinct from that reported for SHH-N co-receptors. Our biochemical assays show that SHH-N may use another interface, one that is required for its co-receptor binding, to recruit PTCH1 in the absence of a covalently attached palmitate. Our work provides atomic insights into the recognition of the N-terminal domain of HH (HH-N) by PTCH1, offers a structural basis for cooperative binding of HH-N to various receptors and serves as a molecular framework for HH signalling and its malfunction in disease.

PubMed: 29995851
DOI: 10.1038/s41586-018-0308-7

実験手法

ELECTRON MICROSCOPY (3.8 Å)