6OEP

Cryo-EM structure of mouse RAG1/2 12RSS-NFC/23RSS-PRC complex (DNA1)

6OEP の概要

• エントリーDOI: 10.2210/pdb6oep/pdb
• 関連するPDBエントリー: 6OEM 6OEN 6OEO
• EMDBエントリー: 20030 20031 20032 20033
• 分子名称: V(D)J recombination-activating protein 1, V(D)J recombination-activating protein 2, DNA (46-MER), ... (8 entities in total)
• 機能のキーワード: v(d)j recombination, dna transposition, rag, scid, recombination, recombination-dna complex, recombination/dna
• 由来する生物種: Mus musculus (Mouse); 詳細
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 425710.43

構造登録者

Chen, X.,Cui, Y.,Zhou, Z.H.,Yang, W.,Gellert, M. (登録日: 2019-03-27, 公開日: 2020-01-29, 最終更新日: 2025-05-21)

主引用文献

Chen, X.,Cui, Y.,Best, R.B.,Wang, H.,Zhou, Z.H.,Yang, W.,Gellert, M.
Cutting antiparallel DNA strands in a single active site.
Nat.Struct.Mol.Biol., 27:119-126, 2020

PubMed Abstract: A single enzyme active site that catalyzes multiple reactions is a well-established biochemical theme, but how one nuclease site cleaves both DNA strands of a double helix has not been well understood. In analyzing site-specific DNA cleavage by the mammalian RAG1-RAG2 recombinase, which initiates V(D)J recombination, we find that the active site is reconfigured for the two consecutive reactions and the DNA double helix adopts drastically different structures. For initial nicking of the DNA, a locally unwound and unpaired DNA duplex forms a zipper via alternating interstrand base stacking, rather than melting as generally thought. The second strand cleavage and formation of a hairpin-DNA product requires a global scissor-like movement of protein and DNA, delivering the scissile phosphate into the rearranged active site.

PubMed: 32015552
DOI: 10.1038/s41594-019-0363-2

実験手法

ELECTRON MICROSCOPY (3.7 Å)