6OEC

Yeast Spc42 Trimeric Coiled-Coil Amino Acids 181-211 fused to PDB: 3H5I

6OEC の概要

• エントリーDOI: 10.2210/pdb6oec/pdb
• 分子名称: Response regulator/sensory box protein/GGDEF domain protein,Spindle pole body component SPC42, CALCIUM ION (3 entities in total)
• 機能のキーワード: yeast centrosome, spindle pole body, trimeric coiled-coil, structural protein
• 由来する生物種: Carboxydothermus hydrogenoformans (strain ATCC BAA-161 / DSM 6008 / Z-2901); 詳細
• タンパク質・核酸の鎖数: 12
• 化学式量合計: 214029.63

構造登録者

Drennan, A.C.,Shivaani, K.,Seeger, M.A.,Andreas, M.P.,Gardner, J.M.,Sether, E.K.R.,Jasperson, S.L.,Rayment, I. (登録日: 2019-03-27, 公開日: 2019-04-24, 最終更新日: 2023-10-11)

主引用文献

Drennan, A.C.,Krishna, S.,Seeger, M.A.,Andreas, M.P.,Gardner, J.M.,Sether, E.K.R.,Jaspersen, S.L.,Rayment, I.
Structure and function of Spc42 coiled-coils in yeast centrosome assembly and duplication.
Mol.Biol.Cell, 30:1505-1522, 2019

PubMed Abstract: Centrosomes and spindle pole bodies (SPBs) are membraneless organelles whose duplication and assembly is necessary for bipolar mitotic spindle formation. The structural organization and functional roles of major proteins in these organelles can provide critical insights into cell division control. Spc42, a phosphoregulated protein with an N-terminal dimeric coiled-coil (DCC), assembles into a hexameric array at the budding yeast SPB core, where it functions as a scaffold for SPB assembly. Here, we present in vitro and in vivo data to elucidate the structural arrangement and biological roles of Spc42 elements. Crystal structures reveal details of two additional coiled-coils in Spc42: a central trimeric coiled-coil and a C-terminal antiparallel DCC. Contributions of the three Spc42 coiled-coils and adjacent undetermined regions to the formation of an ∼145 Å hexameric lattice in an in vitro lipid monolayer assay and to SPB duplication and assembly in vivo reveal structural and functional redundancy in Spc42 assembly. We propose an updated model that incorporates the inherent symmetry of these Spc42 elements into a lattice, and thereby establishes the observed sixfold symmetry. The implications of this model for the organization of the central SPB core layer are discussed.

PubMed: 30969903
DOI: 10.1091/mbc.E19-03-0167

実験手法

X-RAY DIFFRACTION (2.514 Å)