6OCR

Crystal structure of human KCTD16 T1 domain

6OCR の概要

• エントリーDOI: 10.2210/pdb6ocr/pdb
• 分子名称: BTB/POZ domain-containing protein KCTD16 (2 entities in total)
• 機能のキーワード: kctd16, pentamer, gaba(b) receptor, signaling protein
• 由来する生物種: Homo sapiens (Human)
• タンパク質・核酸の鎖数: 15
• 化学式量合計: 200493.63

構造登録者

Zuo, H.,Glaaser, I.,Zhao, Y.,Kurinov, I.,Mosyak, L.,Wang, H.,Liu, J.,Park, J.,Frangaj, A.,Sturchler, E.,Zhou, M.,McDonald, P.,Geng, Y.,Slesinger, P.A.,Fan, Q.R. (登録日: 2019-03-25, 公開日: 2019-04-10, 最終更新日: 2024-03-13)

主引用文献

Zuo, H.,Glaaser, I.,Zhao, Y.,Kurinov, I.,Mosyak, L.,Wang, H.,Liu, J.,Park, J.,Frangaj, A.,Sturchler, E.,Zhou, M.,McDonald, P.,Geng, Y.,Slesinger, P.A.,Fan, Q.R.
Structural basis for auxiliary subunit KCTD16 regulation of the GABABreceptor.
Proc.Natl.Acad.Sci.USA, 116:8370-8379, 2019

PubMed Abstract: Metabotropic GABA receptors mediate a significant fraction of inhibitory neurotransmission in the brain. Native GABA receptor complexes contain the principal subunits GABA and GABA, which form an obligate heterodimer, and auxiliary subunits, known as potassium channel tetramerization domain-containing proteins (KCTDs). KCTDs interact with GABA receptors and modify the kinetics of GABA receptor signaling. Little is known about the molecular mechanism governing the direct association and functional coupling of GABA receptors with these auxiliary proteins. Here, we describe the high-resolution structure of the KCTD16 oligomerization domain in complex with part of the GABA receptor. A single GABA C-terminal peptide is bound to the interior of an open pentamer formed by the oligomerization domain of five KCTD16 subunits. Mutation of specific amino acids identified in the structure of the GABA-KCTD16 interface disrupted both the biochemical association and functional modulation of GABA receptors and G protein-activated inwardly rectifying K channel (GIRK) channels. These interfacial residues are conserved among KCTDs, suggesting a common mode of KCTD interaction with GABA receptors. Defining the binding interface of GABA receptor and KCTD reveals a potential regulatory site for modulating GABA-receptor function in the brain.

PubMed: 30971491
DOI: 10.1073/pnas.1903024116

実験手法

X-RAY DIFFRACTION (2.28 Å)