6OAJ

HUaE34K 19bp SYM DNA

6OAJ の概要

• エントリーDOI: 10.2210/pdb6oaj/pdb
• 分子名称: DNA-binding protein HU-alpha, DNA (5'-D(P*CP*GP*GP*TP*TP*CP*AP*AP*TP*TP*GP*GP*CP*AP*CP*GP*CP*GP*C)-3'), DNA (5'-D(P*GP*CP*GP*CP*GP*TP*GP*CP*CP*AP*AP*TP*TP*GP*AP*AP*CP*CP*GP*C)-3') (3 entities in total)
• 機能のキーワード: nucleoid associated protein, dna supercoiling, histone like proteins, dna binding protein, dna binding protein-dna complex, dna binding protein/dna
• 由来する生物種: Escherichia coli (strain K12); 詳細
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 50141.90

構造登録者

Remesh, S.G.,Hammel, M. (登録日: 2019-03-16, 公開日: 2020-03-18, 最終更新日: 2023-10-11)

主引用文献

Remesh, S.G.,Verma, S.C.,Chen, J.H.,Ekman, A.A.,Larabell, C.A.,Adhya, S.,Hammel, M.
Nucleoid remodeling during environmental adaptation is regulated by HU-dependent DNA bundling.
Nat Commun, 11:2905-2905, 2020

PubMed Abstract: Bacterial nucleoid remodeling dependent on conserved histone-like protein, HU is one of the determining factors in global gene regulation. By imaging of near-native, unlabeled E. coli cells by soft X-ray tomography, we show that HU remodels nucleoids by promoting the formation of a dense condensed core surrounded by less condensed isolated domains. Nucleoid remodeling during cell growth and environmental adaptation correlate with pH and ionic strength controlled molecular switch that regulated HUαα dependent intermolecular DNA bundling. Through crystallographic and solution-based studies we show that these effects mechanistically rely on HUαα promiscuity in forming multiple electrostatically driven multimerization interfaces. Changes in DNA bundling consequently affects gene expression globally, likely by constrained DNA supercoiling. Taken together our findings unveil a critical function of HU-DNA interaction in nucleoid remodeling that may serve as a general microbial mechanism for transcriptional regulation to synchronize genetic responses during the cell cycle and adapt to changing environments.

PubMed: 32518228
DOI: 10.1038/s41467-020-16724-5

実験手法

X-RAY DIFFRACTION (4.092 Å)