6O9E

Structure of HIV-1 Reverse Transcriptase in complex with DNA and INDOPY-1

6O9E の概要

• エントリーDOI: 10.2210/pdb6o9e/pdb
• 関連するBIRD辞書のPRD_ID: PRD_900003
• 分子名称: Reverse transcriptase p66, TETRAETHYLENE GLYCOL, Reverse transcriptase p51, ... (11 entities in total)
• 機能のキーワード: human immunodeficiency virus 1, protein/dna, nucleotide-competing reverse transcriptase inhibitor, transferase, transferase-dna complex, transferase/dna
• 由来する生物種: Human immunodeficiency virus type 1 (HIV-1); 詳細
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 254104.87

構造登録者

Ruiz, F.X.,Hoang, A.,Das, K.,Arnold, E. (登録日: 2019-03-13, 公開日: 2019-10-23, 最終更新日: 2023-10-11)

主引用文献

Ruiz, F.X.,Hoang, A.,Das, K.,Arnold, E.
Structural Basis of HIV-1 Inhibition by Nucleotide-Competing Reverse Transcriptase Inhibitor INDOPY-1.
J.Med.Chem., 62:9996-10002, 2019

PubMed Abstract: HIV-1 reverse transcriptase (RT) is an essential enzyme, targeting half of approved anti-AIDS drugs. While nucleoside RT inhibitors (NRTIs) are DNA chain terminators, the nucleotide-competing RT inhibitor (NcRTI) INDOPY-1 blocks dNTP binding to RT. Lack of structural information hindered INDOPY-1 improvement. Here we report the HIV-1 RT/DNA/INDOPY-1 crystal structure, revealing a unique mode of inhibitor binding at the polymerase active site without involving catalytic metal ions. The structure may enable new strategies for developing NcRTIs.

PubMed: 31603676
DOI: 10.1021/acs.jmedchem.9b01289

実験手法

X-RAY DIFFRACTION (2.4 Å)