6O8N

Crystal Structure of C9S tetrasulfide state of Sulfide-responsive transcriptional repressor (SqrR) from Rhodobacter capsulatus.

6O8N の概要

• エントリーDOI: 10.2210/pdb6o8n/pdb
• 分子名称: Transcriptional regulator, ArsR family, SULFATE ION (3 entities in total)
• 機能のキーワード: transcription factor, sulfide sensor, reactive sulfur species, photosynthesis regulation, transcription
• 由来する生物種: Rhodobacter capsulatus
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 25104.22

構造登録者

Capdevila, D.A.,Gonzalez-Gutierrez, G.,Giedroc, D.P. (登録日: 2019-03-11, 公開日: 2020-04-01, 最終更新日: 2023-11-15)

主引用文献

Capdevila, D.A.,Walsh, B.J.C.,Zhang, Y.,Dietrich, C.,Gonzalez-Gutierrez, G.,Giedroc, D.P.
Structural basis for persulfide-sensing specificity in a transcriptional regulator.
Nat.Chem.Biol., 17:65-70, 2021

PubMed Abstract: Cysteine thiol-based transcriptional regulators orchestrate the coordinated regulation of redox homeostasis and other cellular processes by 'sensing' or detecting a specific redox-active molecule, which in turn activates the transcription of a specific detoxification pathway. The extent to which these sensors are truly specific in cells for a singular class of reactive small-molecule stressors, for example, reactive oxygen or sulfur species, is largely unknown. Here, we report structural and mechanistic insights into the thiol-based transcriptional repressor SqrR, which reacts exclusively with oxidized sulfur species such as persulfides, to yield a tetrasulfide bridge that inhibits DNA operator-promoter binding. Evaluation of crystallographic structures of SqrR in various derivatized states, coupled with the results of a mass spectrometry-based kinetic profiling strategy, suggest that persulfide selectivity is determined by structural frustration of the disulfide form. These findings led to the identification of an uncharacterized repressor from the bacterial pathogen Acinetobacter baumannii as a persulfide sensor.

PubMed: 33106663
DOI: 10.1038/s41589-020-00671-9

実験手法

X-RAY DIFFRACTION (1.95 Å)