6O7C

Crystal structure of the LjCASTOR gating ring in the Ca2+ and K+ state

6O7C の概要

• エントリーDOI: 10.2210/pdb6o7c/pdb
• 分子名称: Ion channel CASTOR, CALCIUM ION, MAGNESIUM ION, ... (6 entities in total)
• 機能のキーワード: calcium channel, rck domain, gating ring, castor, membrane protein, transport protein
• 由来する生物種: Lotus japonicus
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 60743.91

構造登録者

Jiang, Y.,Kim, S. (登録日: 2019-03-07, 公開日: 2019-09-11, 最終更新日: 2024-03-13)

主引用文献

Kim, S.,Zeng, W.,Bernard, S.,Liao, J.,Venkateshwaran, M.,Ane, J.M.,Jiang, Y.
Ca2+-regulated Ca2+channels with an RCK gating ring control plant symbiotic associations.
Nat Commun, 10:3703-3703, 2019

PubMed Abstract: A family of plant nuclear ion channels, including DMI1 (Does not Make Infections 1) and its homologs CASTOR and POLLUX, are required for the establishment of legume-microbe symbioses by generating nuclear and perinuclear Ca spiking. Here we show that CASTOR from Lotus japonicus is a highly selective Ca channel whose activation requires cytosolic/nucleosolic Ca, contrary to the previous suggestion of it being a K channel. Structurally, the cytosolic/nucleosolic ligand-binding soluble region of CASTOR contains two tandem RCK (Regulator of Conductance for K) domains, and four subunits assemble into the gating ring architecture, similar to that of large conductance, Ca-gated K (BK) channels despite the lack of sequence similarity. Multiple ion binding sites are clustered at two locations within each subunit, and three of them are identified to be Ca sites. Our in vitro and in vivo assays also demonstrate the importance of these gating-ring Ca binding sites to the physiological function of CASTOR as well as DMI1.

PubMed: 31420535
DOI: 10.1038/s41467-019-11698-5

実験手法

X-RAY DIFFRACTION (1.85 Å)