6O6X

Crystal structure of Csm6 W14A/E337A mutant in complex with cA4 by cocrystallization

Summary for 6O6X

• Entry DOI: 10.2210/pdb6o6x/pdb
• Descriptor: Csm6, Cyclic RNA cA4 (3 entities in total)
• Functional Keywords: type iii-a crispr-cas system; csm6 w14a-e337a mutant in complex with ca4, immune system, immune system-dna complex, immune system-rna complex, immune system/rna
• Biological source: Thermococcus onnurineus (strain NA1); More
• Total number of polymer chains: 4
• Total formula weight: 101787.63

Authors

Jia, N.,Patel, D.J. (deposition date: 2019-03-07, release date: 2019-07-31, Last modification date: 2024-03-13)

Primary citation

Jia, N.,Jones, R.,Yang, G.,Ouerfelli, O.,Patel, D.J.
CRISPR-Cas III-A Csm6 CARF Domain Is a Ring Nuclease Triggering Stepwise cA4Cleavage with ApA>p Formation Terminating RNase Activity.
Mol.Cell, 75:944-956.e6, 2019

PubMed Abstract: Type III-A CRISPR-Cas surveillance complexes containing multi-subunit Csm effector, guide, and target RNAs exhibit multiple activities, including formation of cyclic-oligoadenylates (cA) from ATP and subsequent cA-mediated cleavage of single-strand RNA (ssRNA) by the trans-acting Csm6 RNase. Our structure-function studies have focused on Thermococcus onnurineus Csm6 to deduce mechanistic insights into how cA binding to the Csm6 CARF domain triggers the RNase activity of the Csm6 HEPN domain and what factors contribute to regulation of RNA cleavage activity. We demonstrate that the Csm6 CARF domain is a ring nuclease, whereby bound cA is stepwise cleaved initially to ApApApA>p and subsequently to ApA>p in its CARF domain-binding pocket, with such cleavage bursts using a timer mechanism to regulate the RNase activity of the Csm6 HEPN domain. In addition, we establish T. onnurineus Csm6 as an adenosine-specific RNase and identify a histidine in the cA CARF-binding pocket involved in autoinhibitory regulation of RNase activity.

PubMed: 31326273
DOI: 10.1016/j.molcel.2019.06.014

Experimental method

X-RAY DIFFRACTION (2.11 Å)