6O6H

RIAM cc-RA-PH structure in the P21212 space group

6O6H の概要

• エントリーDOI: 10.2210/pdb6o6h/pdb
• 分子名称: Amyloid beta A4 precursor protein-binding family B member 1-interacting protein (2 entities in total)
• 機能のキーワード: rap1 effector ra-ph, signaling protein
• 由来する生物種: Mus musculus (Mouse)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 34326.42

構造登録者

Wu, J. (登録日: 2019-03-06, 公開日: 2020-09-02, 最終更新日: 2024-03-13)

主引用文献

Cho, E.A.,Zhang, P.,Kumar, V.,Kavalchuk, M.,Zhang, H.,Huang, Q.,Duncan, J.S.,Wu, J.
Phosphorylation of RIAM by Src Promotes Integrin Activation by Unmasking the PH Domain of RIAM.
Structure, 2020

PubMed Abstract: Integrin activation controls cell adhesion, migration, invasion, and extracellular matrix remodeling. RIAM (RAP1-GTP-interacting adaptor molecule) is recruited by activated RAP1 to the plasma membrane (PM) to mediate integrin activation via an inside-out signaling pathway. This process requires the association of the pleckstrin homology (PH) domain of RIAM with the membrane PIP2. We identify a conserved intermolecular interface that masks the PIP2-binding site in the PH domains of RIAM. Our data indicate that phosphorylation of RIAM by Src family kinases disrupts this PH-mediated interface, unmasks the membrane PIP2-binding site, and promotes integrin activation. We further demonstrate that this process requires phosphorylation of Tyr267 and Tyr427 in the RIAM PH domain by Src. Our data reveal an unorthodox regulatory mechanism of small GTPase effector proteins by phosphorylation-dependent PM association of the PH domain and provide new insights into the link between Src kinases and integrin signaling.

PubMed: 33275877
DOI: 10.1016/j.str.2020.11.011

実験手法

X-RAY DIFFRACTION (2.5 Å)