6O5T

Crystal Structure of VIM-2 with Compound 16

6O5T の概要

• エントリーDOI: 10.2210/pdb6o5t/pdb
• 分子名称: Beta-lactamase class B VIM-2, ZINC ION, [(5,7-dibromo-2-oxo-1,2-dihydroquinolin-4-yl)methyl]phosphonic acid, ... (5 entities in total)
• 機能のキーワード: carbapenemase, phosphonate, inhibitor, complex, hydrolase, hydrolase-inhibitor complex, hydrolase/inhibitor
• 由来する生物種: Pseudomonas aeruginosa
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 53117.47

構造登録者

Akhtar, A.,Chen, Y. (登録日: 2019-03-04, 公開日: 2019-09-25, 最終更新日: 2023-10-11)

主引用文献

Pemberton, O.A.,Jaishankar, P.,Akhtar, A.,Adams, J.L.,Shaw, L.N.,Renslo, A.R.,Chen, Y.
Heteroaryl Phosphonates as Noncovalent Inhibitors of Both Serine- and Metallocarbapenemases.
J.Med.Chem., 62:8480-8496, 2019

PubMed Abstract: Gram-negative pathogens expressing serine β-lactamases (SBLs) and metallo-β-lactamases (MBLs), especially those with carbapenemase activity, threaten the clinical utility of almost all β-lactam antibiotics. Here we describe the discovery of a heteroaryl phosphonate scaffold that exhibits noncovalent cross-class inhibition of representative carbapenemases, specifically the SBL KPC-2 and the MBLs NDM-1 and VIM-2. The most potent lead, compound , exhibited low nM to low μM inhibition of KPC-2, NDM-1, and VIM-2. Compound potentiated imipenem efficacy against resistant clinical and laboratory bacterial strains expressing carbapenemases while showing some cytotoxicity toward human HEK293T cells only at concentrations above 100 μg/mL. Complex structures with KPC-2, NDM-1, and VIM-2 demonstrate how these inhibitors achieve high binding affinity to both enzyme classes. These findings provide a structurally and mechanistically new scaffold for drug discovery targeting multidrug resistant Gram-negative pathogens and more generally highlight the active site features of carbapenemases that can be leveraged for lead discovery.

PubMed: 31483651
DOI: 10.1021/acs.jmedchem.9b00728

実験手法

X-RAY DIFFRACTION (2.1 Å)