6O58

Human MCU-EMRE complex, dimer of channel

6O58 の概要

• エントリーDOI: 10.2210/pdb6o58/pdb
• EMDBエントリー: 0625 0626 0627
• 分子名称: Calcium uniporter protein, mitochondrial, Essential MCU regulator, mitochondrial, CALCIUM ION (3 entities in total)
• 機能のキーワード: ion channel, complex, membrane protein, transport protein
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 16
• 化学式量合計: 411079.90

構造登録者

Wang, Y.,Bai, X.,Jiang, Y. (登録日: 2019-03-01, 公開日: 2019-05-22, 最終更新日: 2024-03-20)

主引用文献

Wang, Y.,Nguyen, N.X.,She, J.,Zeng, W.,Yang, Y.,Bai, X.C.,Jiang, Y.
Structural Mechanism of EMRE-Dependent Gating of the Human Mitochondrial Calcium Uniporter.
Cell, 177:1252-, 2019

PubMed Abstract: Mitochondrial calcium uptake is crucial to the regulation of eukaryotic Ca homeostasis and is mediated by the mitochondrial calcium uniporter (MCU). While MCU alone can transport Ca in primitive eukaryotes, metazoans require an essential single membrane-spanning auxiliary component called EMRE to form functional channels; however, the molecular mechanism of EMRE regulation remains elusive. Here, we present the cryo-EM structure of the human MCU-EMRE complex, which defines the interactions between MCU and EMRE as well as pinpoints the juxtamembrane loop of MCU and extended linker of EMRE as the crucial elements in the EMRE-dependent gating mechanism among metazoan MCUs. The structure also features the dimerization of two MCU-EMRE complexes along an interface at the N-terminal domain (NTD) of human MCU that is a hotspot for post-translational modifications. Thus, the human MCU-EMRE complex, which constitutes the minimal channel components among metazoans, provides a framework for future mechanistic studies on MCU.

PubMed: 31080062
DOI: 10.1016/j.cell.2019.03.050

実験手法

ELECTRON MICROSCOPY (3.8 Å)