6O3W

Crystal structure of yeast Nrd1 CID in complex with Sen1 NIM1

6O3W の概要

• エントリーDOI: 10.2210/pdb6o3w/pdb
• 関連するPDBエントリー: 6O3X 6O3Y
• 分子名称: Helicase SEN1, Protein NRD1 (3 entities in total)
• 機能のキーワード: complex, cid, ctd mimic, hydrolase
• 由来する生物種: Saccharomyces cerevisiae (Baker's yeast); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 42037.42

構造登録者

Zhang, Y.,Tong, L. (登録日: 2019-02-27, 公開日: 2019-06-05, 最終更新日: 2023-10-11)

主引用文献

Zhang, Y.,Chun, Y.,Buratowski, S.,Tong, L.
Identification of Three Sequence Motifs in the Transcription Termination Factor Sen1 that Mediate Direct Interactions with Nrd1.
Structure, 27:1156-1161.e4, 2019

PubMed Abstract: The Nrd1-Nab3-Sen1 (NNS) complex carries out the transcription termination of non-coding RNAs (ncRNAs) by RNA polymerase II (Pol II) in yeast, although the detailed interactions among its subunits remain obscure. Here we have identified three sequence motifs in Sen1 that mediate direct interactions with the Pol II CTD interaction domain (CID) of Nrd1, determined the crystal structures of these Nrd1 interaction motifs (NIMs) bound to the CID, and characterized the interactions in vitro and in yeast. Removal of all three NIMs abolishes NNS complex formation and gives rise to ncRNA termination defects.

PubMed: 31104813
DOI: 10.1016/j.str.2019.04.005

実験手法

X-RAY DIFFRACTION (2.1 Å)