6O0Y
Conformational states of Cas9-sgRNA-DNA ternary complex in the presence of magnesium
Summary for 6O0Y
Entry DOI | 10.2210/pdb6o0y/pdb |
EMDB information | 0584 |
Descriptor | CRISPR-associated endonuclease Cas9/Csn1, single guide RNA, 5' product of target strand DNA, ... (5 entities in total) |
Functional Keywords | crispr, cas9, nuclease, genome editing, hydrolase, hydrolase-rna-dna complex, hydrolase/rna/dna |
Biological source | Streptococcus pyogenes More |
Total number of polymer chains | 5 |
Total formula weight | 216628.65 |
Authors | Zhu, X.,Clarke, R.,Puppala, A.K.,Chittori, S.,Merk, A.,Merrill, B.J.,Simonovic, M.,Subramaniam, S. (deposition date: 2019-02-17, release date: 2019-07-10, Last modification date: 2024-03-20) |
Primary citation | Zhu, X.,Clarke, R.,Puppala, A.K.,Chittori, S.,Merk, A.,Merrill, B.J.,Simonovic, M.,Subramaniam, S. Cryo-EM structures reveal coordinated domain motions that govern DNA cleavage by Cas9. Nat.Struct.Mol.Biol., 26:679-685, 2019 Cited by PubMed: 31285607DOI: 10.1038/s41594-019-0258-2 PDB entries with the same primary citation |
Experimental method | ELECTRON MICROSCOPY (3.37 Å) |
Structure validation
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