6O09

Structure of AtPCNA in complex with the PIP motif of ATXR6

6O09 の概要

• エントリーDOI: 10.2210/pdb6o09/pdb
• 分子名称: Proliferating cellular nuclear antigen 1, Uncharacterized protein (3 entities in total)
• 機能のキーワード: histone, methylation, nucleosome, pcna, pip, dna replication, atxr5, atxr6, nuclear protein
• 由来する生物種: Arabidopsis thaliana (Mouse-ear cress); 詳細
• タンパク質・核酸の鎖数: 12
• 化学式量合計: 221987.77

構造登録者

Couture, J.F.,Davarinejad, H. (登録日: 2019-02-15, 公開日: 2019-03-27, 最終更新日: 2024-10-30)

主引用文献

Davarinejad, H.,Joshi, M.,Ait-Hamou, N.,Munro, K.,Couture, J.F.
ATXR5/6 Forms Alternative Protein Complexes with PCNA and the Nucleosome Core Particle.
J. Mol. Biol., 431:1370-1379, 2019

PubMed Abstract: The proliferating cell nuclear antigen (PCNA) is a sliding clamp associated with DNA polymerases and serves as a binding platform for the recruitment of regulatory proteins linked to DNA damage repair, cell cycle regulation, and epigenetic signaling. The histone H3 lysine-27 (H3K27) mono-methyltransferase Arabidopsis trithorax-related protein 5/6 (ATXR5/6) associates with PCNA, and this interaction has been proposed to act as a key determinant controlling the reestablishment of H3K27 mono-methylation following replication. In this study, we provide biochemical evidence showing that PCNA inhibits ATXR6 enzymatic activity. The structure of the ATXR6 PCNA-interacting peptide (PIP) in complex with PCNA indicates that a trio of hydrophobic residues contributes to the binding of the enzyme to the sliding clamp. Finally, despite the presence of three PIP binding clefts, only two molecules of ATXR6 bind to PCNA likely enabling the recruitment of a third protein to the sliding clamp. Collectively, these results rule out the model wherein PCNA-bound ATXR6 actively reestablishes H3K27 mono-methylation following DNA replication and provides insights into the role of ATXR6 PIP motif in its interaction with PCNA.

PubMed: 30826376
DOI: 10.1016/j.jmb.2019.02.020

実験手法

X-RAY DIFFRACTION (2.06 Å)