6NYK

Crystal structure of computationally designed protein XAX_GGDQ

6NYK の概要

• エントリーDOI: 10.2210/pdb6nyk/pdb
• 分子名称: Design construct XAX_GGDQ (1 entity in total)
• 機能のキーワード: homotrimer, helix, de novo protein
• 由来する生物種: synthetic construct
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 33485.45

構造登録者

Wei, K.Y.,Bick, M.J. (登録日: 2019-02-11, 公開日: 2020-04-22, 最終更新日: 2024-03-13)

主引用文献

Wei, K.Y.,Moschidi, D.,Bick, M.J.,Nerli, S.,McShan, A.C.,Carter, L.P.,Huang, P.S.,Fletcher, D.A.,Sgourakis, N.G.,Boyken, S.E.,Baker, D.
Computational design of closely related proteins that adopt two well-defined but structurally divergent folds.
Proc.Natl.Acad.Sci.USA, 117:7208-7215, 2020

PubMed Abstract: The plasticity of naturally occurring protein structures, which can change shape considerably in response to changes in environmental conditions, is critical to biological function. While computational methods have been used for de novo design of proteins that fold to a single state with a deep free-energy minimum [P.-S. Huang, S. E. Boyken, D. Baker, 537, 320-327 (2016)], and to reengineer natural proteins to alter their dynamics [J. A. Davey, A. M. Damry, N. K. Goto, R. A. Chica, 13, 1280-1285 (2017)] or fold [P. A. Alexander, Y. He, Y. Chen, J. Orban, P. N. Bryan, 106, 21149-21154 (2009)], the de novo design of closely related sequences which adopt well-defined but structurally divergent structures remains an outstanding challenge. We designed closely related sequences (over 94% identity) that can adopt two very different homotrimeric helical bundle conformations-one short (∼66 Å height) and the other long (∼100 Å height)-reminiscent of the conformational transition of viral fusion proteins. Crystallographic and NMR spectroscopic characterization shows that both the short- and long-state sequences fold as designed. We sought to design bistable sequences for which both states are accessible, and obtained a single designed protein sequence that populates either the short state or the long state depending on the measurement conditions. The design of sequences which are poised to adopt two very different conformations sets the stage for creating large-scale conformational switches between structurally divergent forms.

PubMed: 32188784
DOI: 10.1073/pnas.1914808117

実験手法

X-RAY DIFFRACTION (2.8 Å)