6NWD

X-ray Crystallographic structure of Gloeobacter rhodopsin

6NWD の概要

• エントリーDOI: 10.2210/pdb6nwd/pdb
• 分子名称: Gll0198 protein, RETINAL, 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE, ... (6 entities in total)
• 機能のキーワード: proton pump, photoreceptor, transport protein
• 由来する生物種: Gloeobacter violaceus (strain ATCC 29082 / PCC 7421)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 34941.39

構造登録者

Ernst, O.P.,Morizumi, T.,Ou, W.L. (登録日: 2019-02-06, 公開日: 2019-08-14, 最終更新日: 2024-10-23)

主引用文献

Morizumi, T.,Ou, W.L.,Van Eps, N.,Inoue, K.,Kandori, H.,Brown, L.S.,Ernst, O.P.
X-ray Crystallographic Structure and Oligomerization of Gloeobacter Rhodopsin.
Sci Rep, 9:11283-11283, 2019

PubMed Abstract: Gloeobacter rhodopsin (GR) is a cyanobacterial proton pump which can be potentially applied to optogenetics. We solved the crystal structure of GR and found that it has overall similarity to the homologous proton pump from Salinibacter ruber, xanthorhodopsin (XR). We identified distinct structural characteristics of GR's hydrogen bonding network in the transmembrane domain as well as the displacement of extracellular sides of the transmembrane helices relative to those of XR. Employing Raman spectroscopy and flash-photolysis, we found that GR in the crystals exists in a state which displays retinal conformation and photochemical cycle similar to the functional form observed in lipids. Based on the crystal structure of GR, we selected a site for spin labeling to determine GR's oligomerization state using double electron-electron resonance (DEER) spectroscopy and demonstrated the pH-dependent pentamer formation of GR. Determination of the structure of GR as well as its pentamerizing propensity enabled us to reveal the role of structural motifs (extended helices, 3-omega motif and flipped B-C loop) commonly found among light-driven bacterial pumps in oligomer formation. Here we propose a new concept to classify these pumps based on the relationship between their oligomerization propensities and these structural determinants.

PubMed: 31375689
DOI: 10.1038/s41598-019-47445-5

実験手法

X-RAY DIFFRACTION (2 Å)