6NUW

Yeast Ctf19 complex

6NUW の概要

• エントリーDOI: 10.2210/pdb6nuw/pdb
• EMDBエントリー: 0523
• 分子名称: Inner kinetochore subunit IML3, Inner kinetochore subunit Mcm22, Inner kinetochore subunit Mcm16, ... (12 entities in total)
• 機能のキーワード: kinetochore, cell cycle
• 由来する生物種: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast); 詳細
• タンパク質・核酸の鎖数: 13
• 化学式量合計: 493093.15

構造登録者

Hinshaw, S.M.,Harrison, S.C. (登録日: 2019-02-03, 公開日: 2019-04-03, 最終更新日: 2024-03-20)

主引用文献

Hinshaw, S.M.,Harrison, S.C.
The structure of the Ctf19c/CCAN from budding yeast.
Elife, 8:-, 2019

PubMed Abstract: Eukaryotic kinetochores connect spindlemicrotubules to chromosomal centromeres. A group of proteins called the Ctf19 complex (Ctf19c) in yeast and the constitutive centromere associated network (CCAN) in other organisms creates the foundation of a kinetochore. The Ctf19c/CCAN influences the timing of kinetochore assembly, sets its location by associating with a specialized nucleosome containing the histone H3 variant Cse4/CENP-A, and determines the organization of the microtubule attachment apparatus. We present here the structure of a reconstituted 13-subunit Ctf19c determined by cryo-electron microscopy at ~4 Å resolution. The structure accounts for known and inferred contacts with the Cse4 nucleosome and for an observed assembly hierarchy. We describe its implications for establishment of kinetochores and for their regulation by kinases throughout the cell cycle.

PubMed: 30762520
DOI: 10.7554/eLife.44239

実験手法

ELECTRON MICROSCOPY (4.25 Å)