6NKO

Crystal structure of ForH

Summary for 6NKO

• Entry DOI: 10.2210/pdb6nko/pdb
• Descriptor: ForH (2 entities in total)
• Functional Keywords: apo structure, formycin a, unknown function
• Biological source: Streptomyces kaniharaensis
• Total number of polymer chains: 4
• Total formula weight: 92769.56

Authors

Zheng, J.,Irani, S.,Zhang, Y. (deposition date: 2019-01-07, release date: 2019-04-17, Last modification date: 2023-10-11)

Primary citation

Wang, S.A.,Ko, Y.,Zeng, J.,Geng, Y.,Ren, D.,Ogasawara, Y.,Irani, S.,Zhang, Y.,Liu, H.W.
Identification of the Formycin A Biosynthetic Gene Cluster from Streptomyces kaniharaensis Illustrates the Interplay between Biological Pyrazolopyrimidine Formation and de Novo Purine Biosynthesis.
J. Am. Chem. Soc., 141:6127-6131, 2019

PubMed Abstract: Formycin A is a potent purine nucleoside antibiotic with a C-glycosidic linkage between the ribosyl moiety and the pyrazolopyrimidine base. Herein, a cosmid is identified from the Streptomyces kaniharaensis genome library that contains the for gene cluster responsible for the biosynthesis of formycin. Subsequent gene deletion experiments and in vitro characterization of the forBCH gene products established their catalytic functions in formycin biosynthesis. Results also demonstrated that PurH from de novo purine biosynthesis plays a key role in pyrazolopyrimidine formation during biosynthesis of formycin A. The participation of PurH in both pathways represents a good example of how primary and secondary metabolism are interlinked.

PubMed: 30942582
DOI: 10.1021/jacs.9b00241

Experimental method

X-RAY DIFFRACTION (2.403 Å)