6NKO

Crystal structure of ForH

6NKO の概要

• エントリーDOI: 10.2210/pdb6nko/pdb
• 分子名称: ForH (2 entities in total)
• 機能のキーワード: apo structure, formycin a, unknown function
• 由来する生物種: Streptomyces kaniharaensis
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 92769.56

構造登録者

Zheng, J.,Irani, S.,Zhang, Y. (登録日: 2019-01-07, 公開日: 2019-04-17, 最終更新日: 2023-10-11)

主引用文献

Wang, S.A.,Ko, Y.,Zeng, J.,Geng, Y.,Ren, D.,Ogasawara, Y.,Irani, S.,Zhang, Y.,Liu, H.W.
Identification of the Formycin A Biosynthetic Gene Cluster from Streptomyces kaniharaensis Illustrates the Interplay between Biological Pyrazolopyrimidine Formation and de Novo Purine Biosynthesis.
J. Am. Chem. Soc., 141:6127-6131, 2019

PubMed Abstract: Formycin A is a potent purine nucleoside antibiotic with a C-glycosidic linkage between the ribosyl moiety and the pyrazolopyrimidine base. Herein, a cosmid is identified from the Streptomyces kaniharaensis genome library that contains the for gene cluster responsible for the biosynthesis of formycin. Subsequent gene deletion experiments and in vitro characterization of the forBCH gene products established their catalytic functions in formycin biosynthesis. Results also demonstrated that PurH from de novo purine biosynthesis plays a key role in pyrazolopyrimidine formation during biosynthesis of formycin A. The participation of PurH in both pathways represents a good example of how primary and secondary metabolism are interlinked.

PubMed: 30942582
DOI: 10.1021/jacs.9b00241

実験手法

X-RAY DIFFRACTION (2.403 Å)