6NJY

Type IV CRISPR associated RNA endonuclease Cas6 - apo form

6NJY の概要

• エントリーDOI: 10.2210/pdb6njy/pdb
• 分子名称: Type IV CRISPR associated Cas6 RNA endonuclease, SULFATE ION, beta-D-glucopyranose, ... (4 entities in total)
• 機能のキーワード: crispr, cas, nuclease, rna, cas6, csf5, rna binding protein
• 由来する生物種: Mahella australiensis (strain DSM 15567 / CIP 107919 / 50-1 BON)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 59103.36

構造登録者

Jackson, R.N.,Warner, E.,Olsen, K.J. (登録日: 2019-01-04, 公開日: 2019-07-10, 最終更新日: 2024-03-13)

主引用文献

Taylor, H.N.,Warner, E.E.,Armbrust, M.J.,Crowley, V.M.,Olsen, K.J.,Jackson, R.N.
Structural basis of Type IV CRISPR RNA biogenesis by a Cas6 endoribonuclease.
Rna Biol., 16:1438-1447, 2019

PubMed Abstract: Prokaryotic CRISPR-Cas adaptive immune systems rely on small non-coding RNAs derived from CRISPR loci to recognize and destroy complementary nucleic acids. However, the mechanism of Type IV CRISPR RNA (crRNA) biogenesis is poorly understood. To dissect the mechanism of Type IV CRISPR RNA biogenesis, we determined the x-ray crystal structure of the putative Type IV CRISPR associated endoribonuclease Cas6 from ( Cas6-IV) and characterized its enzymatic activity with RNA cleavage assays. We show that Cas6-IV specifically cleaves Type IV crRNA repeats at the 3' side of a predicted stem loop, with a metal-independent, single-turnover mechanism that relies on a histidine and a tyrosine located within the putative endonuclease active site. Structure and sequence alignments with Cas6 orthologs reveal that although Cas6-IV shares little sequence homology with other Cas6 proteins, all share common structural features that bind distinct crRNA repeat sequences. This analysis of Type IV crRNA biogenesis provides a structural and biochemical framework for understanding the similarities and differences of crRNA biogenesis across multi-subunit Class 1 CRISPR immune systems.

PubMed: 31232162
DOI: 10.1080/15476286.2019.1634965

実験手法

X-RAY DIFFRACTION (1.76 Å)