6NJN

Architecture and subunit arrangement of native AMPA receptors

6NJN の概要

• エントリーDOI: 10.2210/pdb6njn/pdb
• EMDBエントリー: 9389
• 分子名称: Glutamate receptor 1, beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (13 entities in total)
• 機能のキーワード: ampa receptor, ligand gated ion channel, neurotransmitter, synapse, membrane protein
• 由来する生物種: Mus musculus; 詳細
• タンパク質・核酸の鎖数: 15
• 化学式量合計: 675953.86

構造登録者

Gouaux, E.,Zhao, Y. (登録日: 2019-01-03, 公開日: 2019-04-24, 最終更新日: 2021-05-05)

主引用文献

Zhao, Y.,Chen, S.,Swensen, A.C.,Qian, W.J.,Gouaux, E.
Architecture and subunit arrangement of native AMPA receptors elucidated by cryo-EM.
Science, 364:355-362, 2019

PubMed Abstract: Glutamate-gated AMPA receptors mediate the fast component of excitatory signal transduction at chemical synapses throughout all regions of the mammalian brain. AMPA receptors are tetrameric assemblies composed of four subunits, GluA1-GluA4. Despite decades of study, the subunit composition, subunit arrangement, and molecular structure of native AMPA receptors remain unknown. Here we elucidate the structures of 10 distinct native AMPA receptor complexes by single-particle cryo-electron microscopy (cryo-EM). We find that receptor subunits are arranged nonstochastically, with the GluA2 subunit preferentially occupying the B and D positions of the tetramer and with triheteromeric assemblies comprising a major population of native AMPA receptors. Cryo-EM maps define the structure for S2-M4 linkers between the ligand-binding and transmembrane domains, suggesting how neurotransmitter binding is coupled to ion channel gating.

PubMed: 30975770
DOI: 10.1126/science.aaw8250

実験手法

ELECTRON MICROSCOPY (6.5 Å)