6NE0

Structure of double-stranded target DNA engaged Csy complex from Pseudomonas aeruginosa (PA-14)

Replaces:  6MPU

Summary for 6NE0

EMDB information9191
DescriptorCRISPR-associated protein Csy1, CRISPR-associated protein Csy2, CRISPR-associated protein Csy3, ... (7 entities in total)
Functional Keywordstype i-f crispr rna-guided surveillance complex, viral protein mimic, csy-complex, immune system-rna complex, immune system/rna
Biological sourcePseudomonas aeruginosa UCBPP-PA14
Total number of polymer chains12
Total molecular weight375916.48
Authors
Chowdhury, S.,Rollins, M.F.,Carter, J.,Golden, S.M.,Miettinen, H.M.,Santiago-Frangos, A.,Faith, D.,Lawrence, M.C.,Wiedenheft, B.,Lander, G.C. (deposition date: 2018-12-15, release date: 2018-12-26, Last modification date: 2019-04-17)
Primary citation
Rollins, M.F.,Chowdhury, S.,Carter, J.,Golden, S.M.,Miettinen, H.M.,Santiago-Frangos, A.,Faith, D.,Lawrence, C.M.,Lander, G.C.,Wiedenheft, B.
Structure Reveals a Mechanism of CRISPR-RNA-Guided Nuclease Recruitment and Anti-CRISPR Viral Mimicry.
Mol. Cell, 74:132-142.e5, 2019
PubMed: 30872121 (PDB entries with the same primary citation)
DOI: 10.1016/j.molcel.2019.02.001
MImport into Mendeley
Experimental method
ELECTRON MICROSCOPY (3.4 Å)
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Structure validation

ClashscoreRamachandran outliersSidechain outliersRNA backbone700 0.13MetricValuePercentile RanksWorseBetterPercentile relative to all structuresPercentile relative to all EM structures