6NCT

Structure of p110alpha/niSH2 - vector data collection

6NCT の概要

• エントリーDOI: 10.2210/pdb6nct/pdb
• 関連するPDBエントリー: 4OVU
• 分子名称: Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform, Phosphatidylinositol 3-kinase regulatory subunit alpha, TRIS-HYDROXYMETHYL-METHYL-AMMONIUM, ... (4 entities in total)
• 機能のキーワード: pi3k, kinase, phosphatidylinositol 4, 5-bisphosphate 3-kinase alpha isoform, p110, p85, transferase
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 162022.01

構造登録者

Miller, M.S.,Maheshwari, S.,Amzel, L.M.,Gabelli, S.B. (登録日: 2018-12-12, 公開日: 2019-02-06, 最終更新日: 2023-10-11)

主引用文献

Miller, M.S.,Maheshwari, S.,Shi, W.,Gao, Y.,Chu, N.,Soares, A.S.,Cole, P.A.,Amzel, L.M.,Fuchs, M.R.,Jakoncic, J.,Gabelli, S.B.
Getting the Most Out of Your Crystals: Data Collection at the New High-Flux, Microfocus MX Beamlines at NSLS-II.
Molecules, 24:-, 2019

PubMed Abstract: Advances in synchrotron technology are changing the landscape of macromolecular crystallography. The two recently opened beamlines at NSLS-II-AMX and FMX-deliver high-flux microfocus beams that open new possibilities for crystallographic data collection. They are equipped with state-of-the-art experimental stations and automation to allow data collection on previously intractable crystals. Optimized data collection strategies allow users to tailor crystal positioning to optimally distribute the X-ray dose over its volume. Vector data collection allows the user to define a linear trajectory along a well diffracting volume of the crystal and perform rotational data collection while moving along the vector. This is particularly well suited to long, thin crystals. We describe vector data collection of three proteins-Akt1, PI3Kα, and CDP-Chase-to demonstrate its application and utility. For smaller crystals, we describe two methods for multicrystal data collection in a single loop, either manually selecting multiple centers (using H108A-PHM as an example), or "raster-collect", a more automated approach for a larger number of crystals (using CDP-Chase as an example).

PubMed: 30704096
DOI: 10.3390/molecules24030496

実験手法

X-RAY DIFFRACTION (3.35 Å)