6NCP

Crystal structure of HIV-1 broadly neutralizing antibody ACS202

6NCP の概要

• エントリーDOI: 10.2210/pdb6ncp/pdb
• 関連するPDBエントリー: 6NC2 6NC3
• EMDBエントリー: 0433 0434
• 分子名称: ACS202 Fab heavy chain, ACS202 Fab light chain, HIV-1 Fusion Peptide (residues 512-520), ... (7 entities in total)
• 機能のキーワード: antibody, hiv, envelope glycoprotein, fusion peptide, immune system
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 99449.40

構造登録者

Yuan, M.,Wilson, I.A. (登録日: 2018-12-11, 公開日: 2019-06-19, 最終更新日: 2024-10-16)

主引用文献

Yuan, M.,Cottrell, C.A.,Ozorowski, G.,van Gils, M.J.,Kumar, S.,Wu, N.C.,Sarkar, A.,Torres, J.L.,de Val, N.,Copps, J.,Moore, J.P.,Sanders, R.W.,Ward, A.B.,Wilson, I.A.
Conformational Plasticity in the HIV-1 Fusion Peptide Facilitates Recognition by Broadly Neutralizing Antibodies.
Cell Host Microbe, 25:873-883.e5, 2019

PubMed Abstract: The fusion peptide (FP) of HIV-1 envelope glycoprotein (Env) is essential for mediating viral entry. Detection of broadly neutralizing antibodies (bnAbs) that interact with the FP has revealed it as a site of vulnerability. We delineate X-ray and cryo-electron microscopy (cryo-EM) structures of bnAb ACS202, from an HIV-infected elite neutralizer, with an FP and with a soluble Env trimer (AMC011 SOSIP.v4.2) derived from the same patient. We show that ACS202 CDRH3 forms a "β strand" interaction with the exposed hydrophobic FP and recognizes a continuous region of gp120, including a conserved N-linked glycan at N88. A cryo-EM structure of another previously identified bnAb VRC34.01 with AMC011 SOSIP.v4.2 shows that it also penetrates through glycans to target the FP. We further demonstrate that the FP can twist and present different conformations for recognition by bnAbs, which enables approach to Env from diverse angles. The variable recognition of FP by bnAbs thus provides insights for vaccine design.

PubMed: 31194940
DOI: 10.1016/j.chom.2019.04.011

実験手法

X-RAY DIFFRACTION (2.76 Å)