6NCL

Near-atomic structure of icosahedrally averaged PBCV-1 capsid

これはPDB形式変換不可エントリーです。

6NCL の概要

• エントリーDOI: 10.2210/pdb6ncl/pdb
• EMDBエントリー: 0436
• 分子名称: P14, P2, P4, ... (15 entities in total)
• 機能のキーワード: tape-measure protein, minor capsid proteins, zip protein, giant virus, virus
• 由来する生物種: Paramecium bursaria Chlorella virus 1 (PBCV-1); 詳細
• タンパク質・核酸の鎖数: 115
• 化学式量合計: 4793078.58

構造登録者

Fang, Q.,Rossmann, M.G. (登録日: 2018-12-11, 公開日: 2019-01-30, 最終更新日: 2024-10-16)

主引用文献

Fang, Q.,Zhu, D.,Agarkova, I.,Adhikari, J.,Klose, T.,Liu, Y.,Chen, Z.,Sun, Y.,Gross, M.L.,Van Etten, J.L.,Zhang, X.,Rossmann, M.G.
Near-atomic structure of a giant virus.
Nat Commun, 10:388-388, 2019

PubMed Abstract: Although the nucleocytoplasmic large DNA viruses (NCLDVs) are one of the largest group of viruses that infect many eukaryotic hosts, the near-atomic resolution structures of these viruses have remained unknown. Here we describe a 3.5 Å resolution icosahedrally averaged capsid structure of Paramecium bursaria chlorella virus 1 (PBCV-1). This structure consists of 5040 copies of the major capsid protein, 60 copies of the penton protein and 1800 minor capsid proteins of which there are 13 different types. The minor capsid proteins form a hexagonal network below the outer capsid shell, stabilizing the capsid by binding neighboring capsomers together. The size of the viral capsid is determined by a tape-measure, minor capsid protein of which there are 60 copies in the virion. Homologs of the tape-measure protein and some of the other minor capsid proteins exist in other NCLDVs. Thus, a similar capsid assembly pathway might be used by other NCLDVs.

PubMed: 30674888
DOI: 10.1038/s41467-019-08319-6

実験手法

ELECTRON MICROSCOPY (3.5 Å)