6NC2

AMC011 v4.2 SOSIP Env trimer in complex with fusion peptide targeting antibody ACS202 fragment antigen binding

6NC2 の概要

• エントリーDOI: 10.2210/pdb6nc2/pdb
• EMDBエントリー: 0433
• 分子名称: AMC011 v4.2 SOSIP gp120, AMC011 v4.2 SOSIP gp41, Monoclonal antibody ACS202 fragment antigen binding heavy chain, ... (8 entities in total)
• 機能のキーワード: hiv-1 env, sosip, trimer, broadly neutralizing antibody, fusion peptide, viral protein, immune system
• 由来する生物種: Human immunodeficiency virus 1; 詳細
• タンパク質・核酸の鎖数: 24
• 化学式量合計: 793574.02

構造登録者

Cottrell, C.A.,Ozorowski, G.,Yuan, M.,Copps, J.,Wilson, I.A.,Ward, A.B. (登録日: 2018-12-10, 公開日: 2019-06-19, 最終更新日: 2024-11-06)

主引用文献

Yuan, M.,Cottrell, C.A.,Ozorowski, G.,van Gils, M.J.,Kumar, S.,Wu, N.C.,Sarkar, A.,Torres, J.L.,de Val, N.,Copps, J.,Moore, J.P.,Sanders, R.W.,Ward, A.B.,Wilson, I.A.
Conformational Plasticity in the HIV-1 Fusion Peptide Facilitates Recognition by Broadly Neutralizing Antibodies.
Cell Host Microbe, 25:873-, 2019

PubMed Abstract: The fusion peptide (FP) of HIV-1 envelope glycoprotein (Env) is essential for mediating viral entry. Detection of broadly neutralizing antibodies (bnAbs) that interact with the FP has revealed it as a site of vulnerability. We delineate X-ray and cryo-electron microscopy (cryo-EM) structures of bnAb ACS202, from an HIV-infected elite neutralizer, with an FP and with a soluble Env trimer (AMC011 SOSIP.v4.2) derived from the same patient. We show that ACS202 CDRH3 forms a "β strand" interaction with the exposed hydrophobic FP and recognizes a continuous region of gp120, including a conserved N-linked glycan at N88. A cryo-EM structure of another previously identified bnAb VRC34.01 with AMC011 SOSIP.v4.2 shows that it also penetrates through glycans to target the FP. We further demonstrate that the FP can twist and present different conformations for recognition by bnAbs, which enables approach to Env from diverse angles. The variable recognition of FP by bnAbs thus provides insights for vaccine design.

PubMed: 31194940
DOI: 10.1016/j.chom.2019.04.011

実験手法

ELECTRON MICROSCOPY (5.2 Å)