6N9E

Crystal structure of the Thermus thermophilus 70S ribosome in complex with a short substrate mimic CC-Pmn and bound to mRNA and P-site tRNA at 3.7A resolution

This is a large structure.

Summary for 6N9E

Descriptor23S Ribosomal RNA, 5S Ribosomal RNA, 50S Ribosomal Protein L2, ... (59 entities in total)
Functional Keywordsd-amino acids, ribosome structure, peptidyl transferase center, expanded genetic code, ribosome engineering, ribosome
Biological sourceEscherichia coli
More
Total number of polymer chains110
Total molecular weight4434074.68
Authors
Melnikov, S.V.,Khabibullina, N.F.,Mairhofer, E.,Vargas-Rodriguez, O.,Reynolds, N.M.,Micura, R.,Soll, D.,Polikanov, Y.S. (deposition date: 2018-12-03, release date: 2018-12-12, Last modification date: 2018-12-19)
Primary citation
Melnikov, S.V.,Khabibullina, N.F.,Mairhofer, E.,Vargas-Rodriguez, O.,Reynolds, N.M.,Micura, R.,Soll, D.,Polikanov, Y.S.
Mechanistic insights into the slow peptide bond formation with D-amino acids in the ribosomal active site.
Nucleic Acids Res., 2018
PubMed: 30520988 (PDB entries with the same primary citation)
DOI: 10.1093/nar/gky1211
MImport into Mendeley
Experimental method
X-RAY DIFFRACTION (3.7 Å)
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Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliersRNA backbone 0.27910 0.6% 0.6% 8.9% 0.57MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution

More Asymmetric unit images

Molmil generated image of 6n9e
no rotation
Molmil generated image of 6n9e
rotated about x axis by 90°
Molmil generated image of 6n9e
rotated about y axis by 90°
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