6N7P

S. cerevisiae spliceosomal E complex (UBC4)

6N7P の概要

• エントリーDOI: 10.2210/pdb6n7p/pdb
• EMDBエントリー: 0360
• 分子名称: U1 small nuclear ribonucleoprotein 70 kDa homolog, Pre-mRNA-processing protein PRP40, Small nuclear ribonucleoprotein-associated protein B, ... (22 entities in total)
• 機能のキーワード: pre-mrna splicing, spliceosome, e complex, rna binding protein
• 由来する生物種: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast); 詳細
• タンパク質・核酸の鎖数: 21
• 化学式量合計: 944222.10

構造登録者

Liu, S.,Li, X.,Zhou, Z.H.,Zhao, R. (登録日: 2018-11-27, 公開日: 2019-09-18, 最終更新日: 2024-03-20)

主引用文献

Li, X.,Liu, S.,Zhang, L.,Issaian, A.,Hill, R.C.,Espinosa, S.,Shi, S.,Cui, Y.,Kappel, K.,Das, R.,Hansen, K.C.,Zhou, Z.H.,Zhao, R.
A unified mechanism for intron and exon definition and back-splicing.
Nature, 573:375-380, 2019

PubMed Abstract: The molecular mechanisms of exon definition and back-splicing are fundamental unanswered questions in pre-mRNA splicing. Here we report cryo-electron microscopy structures of the yeast spliceosomal E complex assembled on introns, providing a view of the earliest event in the splicing cycle that commits pre-mRNAs to splicing. The E complex architecture suggests that the same spliceosome can assemble across an exon, and that it either remodels to span an intron for canonical linear splicing (typically on short exons) or catalyses back-splicing to generate circular RNA (on long exons). The model is supported by our experiments, which show that an E complex assembled on the middle exon of yeast EFM5 or HMRA1 can be chased into circular RNA when the exon is sufficiently long. This simple model unifies intron definition, exon definition, and back-splicing through the same spliceosome in all eukaryotes and should inspire experiments in many other systems to understand the mechanism and regulation of these processes.

PubMed: 31485080
DOI: 10.1038/s41586-019-1523-6

実験手法

ELECTRON MICROSCOPY (3.6 Å)