6N4E

hPGDS complexed with a quinoline-3-carboxamide

6N4E の概要

• エントリーDOI: 10.2210/pdb6n4e/pdb
• 分子名称: Hematopoietic prostaglandin D synthase, GLUTATHIONE, 7-(difluoromethoxy)-N-[trans-4-(2-hydroxypropan-2-yl)cyclohexyl]quinoline-3-carboxamide, ... (4 entities in total)
• 機能のキーワード: hydrolase
• 由来する生物種: Homo sapiens (Human)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 24113.62

構造登録者

Shewchuk, L.M.,Ward, P. (登録日: 2018-11-19, 公開日: 2019-03-27, 最終更新日: 2024-03-13)

主引用文献

Deaton, D.N.,Do, Y.,Holt, J.,Jeune, M.R.,Kramer, H.F.,Larkin, A.L.,Orband-Miller, L.A.,Peckham, G.E.,Poole, C.,Price, D.J.,Schaller, L.T.,Shen, Y.,Shewchuk, L.M.,Stewart, E.L.,Stuart, J.D.,Thomson, S.A.,Ward, P.,Wilson, J.W.,Xu, T.,Guss, J.H.,Musetti, C.,Rendina, A.R.,Affleck, K.,Anders, D.,Hancock, A.P.,Hobbs, H.,Hodgson, S.T.,Hutchinson, J.,Leveridge, M.V.,Nicholls, H.,Smith, I.E.D.,Somers, D.O.,Sneddon, H.F.,Uddin, S.,Cleasby, A.,Mortenson, P.N.,Richardson, C.,Saxty, G.
The discovery of quinoline-3-carboxamides as hematopoietic prostaglandin D synthase (H-PGDS) inhibitors.
Bioorg. Med. Chem., 27:1456-1478, 2019

PubMed Abstract: With the goal of discovering more selective anti-inflammatory drugs, than COX inhibitors, to attenuate prostaglandin signaling, a fragment-based screen of hematopoietic prostaglandin D synthase was performed. The 76 crystallographic hits were sorted into similar groups, with the 3-cyano-quinoline 1a (FP IC = 220,000 nM, LE = 0.43) being a potent member of the 6,6-fused heterocyclic cluster. Employing SAR insights gained from structural comparisons of other H-PGDS fragment binding mode clusters, the initial hit 1a was converted into the 70-fold more potent quinoline 1d (IC = 3,100 nM, LE = 0.49). A systematic substitution of the amine moiety of 1d, utilizing structural information and array chemistry, with modifications to improve inhibitor stability, resulted in the identification of the 300-fold more active H-PGDS inhibitor tool compound 1bv (IC = 9.9 nM, LE = 0.42). This selective inhibitor exhibited good murine pharmacokinetics, dose-dependently attenuated PGD production in a mast cell degranulation assay and should be suitable to further explore H-PGDS biology.

PubMed: 30858025
DOI: 10.1016/j.bmc.2019.02.017

実験手法

X-RAY DIFFRACTION (1.65 Å)