6N2X

Anti-HIV-1 Fab 2G12 + Man9 re-refinement

「1OP5」から置き換えられました

6N2X の概要

• エントリーDOI: 10.2210/pdb6n2x/pdb
• 分子名称: Fab 2G12 light chain, Fab 2G12 heavy chain, alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-6)]alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose (3 entities in total)
• 機能のキーワード: antibody, carbohydrate, hiv-1, immune system
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 97950.62

構造登録者

Calarese, D.A.,Stanfield, R.L.,Wilson, I.A. (登録日: 2018-11-14, 公開日: 2018-11-28, 最終更新日: 2024-10-16)

主引用文献

Calarese, D.A.,Scanlan, C.N.,Zwick, M.B.,Deechongkit, S.,Mimura, Y.,Kunert, R.,Zhu, P.,Wormald, M.R.,Stanfield, R.L.,Roux, K.H.,Kelly, J.W.,Rudd, P.M.,Dwek, R.A.,Katinger, H.,Burton, D.R.,Wilson, I.A.
Antibody domain exchange is an immunological solution to carbohydrate cluster recognition.
Science, 300:2065-2071, 2003

PubMed Abstract: Human antibody 2G12 neutralizes a broad range of human immunodeficiency virus type 1 (HIV-1) isolates by binding an unusually dense cluster of carbohydrate moieties on the "silent" face of the gp120 envelope glycoprotein. Crystal structures of Fab 2G12 and its complexes with the disaccharide Manalpha1-2Man and with the oligosaccharide Man9GlcNAc2 revealed that two Fabs assemble into an interlocked VH domain-swapped dimer. Further biochemical, biophysical, and mutagenesis data strongly support a Fab-dimerized antibody as the prevalent form that recognizes gp120. The extraordinary configuration of this antibody provides an extended surface, with newly described binding sites, for multivalent interaction with a conserved cluster of oligomannose type sugars on the surface of gp120. The unique interdigitation of Fab domains within an antibody uncovers a previously unappreciated mechanism for high-affinity recognition of carbohydrate or other repeating epitopes on cell or microbial surfaces.

PubMed: 12829775
DOI: 10.1126/science.1083182

実験手法

X-RAY DIFFRACTION (3 Å)