6MYE

Crystal structure of human Scribble PDZ1 domain in complex with internal PDZ binding motif of Src homology 3 domain-containing guanine nucleotide exchange factor (SGEF)

6MYE の概要

• エントリーDOI: 10.2210/pdb6mye/pdb
• 分子名称: Protein scribble homolog, Rho guanine nucleotide exchange factor 26, peptide, FORMIC ACID, ... (4 entities in total)
• 機能のキーワード: guanyl nucleotide exchange factor, sgef/scribble/dlg1 compelx, cell adhesion, tight junction
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 11182.62

構造登録者

Sun, Y.J.,Hou, T.,Gakhar, L.,Fuentes, E.J. (登録日: 2018-11-01, 公開日: 2019-04-10, 最終更新日: 2024-11-13)

主引用文献

Awadia, S.,Huq, F.,Arnold, T.R.,Goicoechea, S.M.,Sun, Y.J.,Hou, T.,Kreider-Letterman, G.,Massimi, P.,Banks, L.,Fuentes, E.J.,Miller, A.L.,Garcia-Mata, R.
SGEF forms a complex with Scribble and Dlg1 and regulates epithelial junctions and contractility.
J.Cell Biol., 218:2699-2725, 2019

PubMed Abstract: The canonical Scribble polarity complex is implicated in regulation of epithelial junctions and apical polarity. Here, we show that SGEF, a RhoG-specific GEF, forms a ternary complex with Scribble and Dlg1, two members of the Scribble complex. SGEF targets to apical junctions in a Scribble-dependent fashion and functions in the regulation of actomyosin-based contractility and barrier function at tight junctions as well as E-cadherin-mediated formation of adherens junctions. Surprisingly, SGEF does not control the establishment of polarity. However, in 3D cysts, SGEF regulates the formation of a single open lumen. Interestingly, SGEF's nucleotide exchange activity regulates the formation and maintenance of adherens junctions, and in cysts the number of lumens formed, whereas SGEF's scaffolding activity is critical for regulation of actomyosin contractility and lumen opening. We propose that SGEF plays a key role in coordinating junctional assembly and actomyosin contractility by bringing together Scribble and Dlg1 and targeting RhoG activation to cell-cell junctions.

PubMed: 31248911
DOI: 10.1083/jcb.201811114

実験手法

X-RAY DIFFRACTION (1.1 Å)