6MWS

crystal structure of the reduced Grx1 from Saccharomyces cerevisiae

6MWS の概要

• エントリーDOI: 10.2210/pdb6mws/pdb
• 分子名称: Glutaredoxin-1, SULFATE ION (3 entities in total)
• 機能のキーワード: glutaredoxin 1, yeast saccharomyces cerevisiae, oxidoreductase
• 由来する生物種: Saccharomyces cerevisiae S288c (Baker's yeast)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 12901.67

構造登録者

Maghool, S.,Maher, J.M. (登録日: 2018-10-30, 公開日: 2019-05-15, 最終更新日: 2024-11-13)

主引用文献

Maghool, S.,La Fontaine, S.,Maher, M.J.
High-resolution crystal structure of the reduced Grx1 from Saccharomyces cerevisiae.
Acta Crystallogr.,Sect.F, 75:392-396, 2019

PubMed Abstract: Grx1, a cytosolic thiol-disulfide oxidoreductase, actively maintains cellular redox homeostasis using glutathione substrates (reduced, GSH, and oxidized, GSSG). Here, the crystallization of reduced Grx1 from the yeast Saccharomyces cerevisiae (yGrx1) in space group P222 and its structure solution and refinement to 1.22 Å resolution are reported. To study the structure-function relationship of yeast Grx1, the crystal structure of reduced yGrx1 was compared with the existing structures of the oxidized and glutathionylated forms. These comparisons revealed structural differences in the conformations of residues neighbouring the Cys27-Cys30 active site which accompany alterations in the redox status of the protein.

PubMed: 31045569
DOI: 10.1107/S2053230X19003327

実験手法

X-RAY DIFFRACTION (1.22 Å)