6MSF

F6 APTAMER MS2 COAT PROTEIN COMPLEX

6MSF の概要

• エントリーDOI: 10.2210/pdb6msf/pdb
• 分子名称: RNA (5'-R(*CP*CP*AP*CP*AP*GP*UP*CP*AP*CP*UP*GP*GP*G)-3'), RNA (5'-R(*CP*AP*GP*UP*CP*AP*CP*UP*GP*G)-3'), PROTEIN (MS2 PROTEIN CAPSID), ... (4 entities in total)
• 機能のキーワード: viral coat protein-rna complex, rna aptamer, rna stem loop, bacteriophage ms2, icosahedral virus, virus-rna complex, virus/rna
• 由来する生物種: Enterobacterio phage MS2
• 細胞内の位置: Virion (Potential): P03612
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 48854.06

構造登録者

Convery, M.A.,Rowsell, S.,Stonehouse, N.J.,Ellington, A.D.,Hirao, I.,Murray, J.B.,Peabody, D.S.,Phillips, S.E.V.,Stockley, P.G. (登録日: 1998-01-06, 公開日: 1998-07-08, 最終更新日: 2023-08-02)

主引用文献

Convery, M.A.,Rowsell, S.,Stonehouse, N.J.,Ellington, A.D.,Hirao, I.,Murray, J.B.,Peabody, D.S.,Phillips, S.E.,Stockley, P.G.
Crystal structure of an RNA aptamer-protein complex at 2.8 A resolution.
Nat.Struct.Biol., 5:133-139, 1998

PubMed Abstract: The crystal structure, at 2.8 A resolution, of an RNA aptamer bound to bacteriophage MS2 coat protein has been determined. It provides an opportunity to compare the interactions of MS2 coat protein and wild type operator with those of an aptamer, whose secondary structure differs from the wild type RNA in having a three-base loop (compared to a tetraloop) and an additional base pair between this loop and the sequence-specific recognition element in the stem. The RNA binds in the same location on the coat protein as the wild type operator and maintains many of the same RNA-protein interactions. In order to achieve this, the RNA stem loop undergoes a concerted rearrangement of the 3' side while leaving the 5' side and the loop interactions largely unchanged, illustrating the ability of RNA to present similar molecular recognition surfaces from distinct primary and secondary structures.

PubMed: 9461079
DOI: 10.1038/nsb0298-133

実験手法

X-RAY DIFFRACTION (2.8 Å)