6MS1
Crystal structure of the human Scribble PDZ1 domain bound to the PDZ-binding motif of APC
Summary for 6MS1
| Entry DOI | 10.2210/pdb6ms1/pdb |
| Descriptor | Protein scribble homolog, APC C-terminus peptide, 1,2-ETHANEDIOL, ... (4 entities in total) |
| Functional Keywords | scribble, apc, pdz domain, cell polarity, structural protein |
| Biological source | Homo sapiens (Human) More |
| Total number of polymer chains | 4 |
| Total formula weight | 21864.46 |
| Authors | How, J.Y.,Kvansakul, M.,Caria, S.,Humbert, P.O. (deposition date: 2018-10-16, release date: 2019-02-06, Last modification date: 2023-10-11) |
| Primary citation | How, J.Y.,Caria, S.,Humbert, P.O.,Kvansakul, M. Crystal structure of the human Scribble PDZ1 domain bound to the PDZ-binding motif of APC. FEBS Lett., 593:533-542, 2019 Cited by PubMed Abstract: Scribble (SCRIB) is an important adaptor protein that controls the establishment and maintenance of apico-basal cell polarity. To better understand how SCRIB controls cell polarity signalling via its PDZ domains, we investigated human SCRIB interactions with adenomatous polyposis coli (APC). We show that SCRIB PDZ1, PDZ2 and PDZ3 are the major interactors with the APC PDZ-binding motif (PBM), whereas SCRIB PDZ4 does not show detectable binding to APC. We then determined the crystal structure of SCRIB PDZ1 domain bound to the APC PBM. Our findings reveal a previously unreported pattern of interactions between the SCRIB PDZ domain region with the C-terminal PDZ binding motif of APC, where SCRIB PDZ1 domain is the highest affinity site. PubMed: 30659601DOI: 10.1002/1873-3468.13329 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.35 Å) |
Structure validation
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