6MS1

Crystal structure of the human Scribble PDZ1 domain bound to the PDZ-binding motif of APC

6MS1 の概要

• エントリーDOI: 10.2210/pdb6ms1/pdb
• 分子名称: Protein scribble homolog, APC C-terminus peptide, 1,2-ETHANEDIOL, ... (4 entities in total)
• 機能のキーワード: scribble, apc, pdz domain, cell polarity, structural protein
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 21864.46

構造登録者

How, J.Y.,Kvansakul, M.,Caria, S.,Humbert, P.O. (登録日: 2018-10-16, 公開日: 2019-02-06, 最終更新日: 2023-10-11)

主引用文献

How, J.Y.,Caria, S.,Humbert, P.O.,Kvansakul, M.
Crystal structure of the human Scribble PDZ1 domain bound to the PDZ-binding motif of APC.
FEBS Lett., 593:533-542, 2019

PubMed Abstract: Scribble (SCRIB) is an important adaptor protein that controls the establishment and maintenance of apico-basal cell polarity. To better understand how SCRIB controls cell polarity signalling via its PDZ domains, we investigated human SCRIB interactions with adenomatous polyposis coli (APC). We show that SCRIB PDZ1, PDZ2 and PDZ3 are the major interactors with the APC PDZ-binding motif (PBM), whereas SCRIB PDZ4 does not show detectable binding to APC. We then determined the crystal structure of SCRIB PDZ1 domain bound to the APC PBM. Our findings reveal a previously unreported pattern of interactions between the SCRIB PDZ domain region with the C-terminal PDZ binding motif of APC, where SCRIB PDZ1 domain is the highest affinity site.

PubMed: 30659601
DOI: 10.1002/1873-3468.13329

実験手法

X-RAY DIFFRACTION (1.35 Å)